Raw signal of the oxygen sensor: Difference between revisions

Latest revision as of 09:13, 17 April 2024

high-resolution terminology - matching measurements at high-resolution

Raw signal of the oxygen sensor

Description

The raw signal of the polarographic oxygen sensor is the current I_el [µA], 1 µA = 10^-6 C·s^-1, (DatLab 8) or the electric potential difference (voltage) [V], 1 V = 1 J·C^-1, obtained after a current-to-voltage conversion in the O2k (DatLab 7 and previous versions).

Abbreviation: R

Reference: Oxygen signal

MitoPedia O2k and high-resolution respirometry: DatLab, Oroboros QM

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-{{Technical support}}
+{{MitoPedia
+|abbr=R
-:::: The raw signal of the [[polarographic oxygen sensor]] (POS) depends on the [[gain (O2 channel)|gain]] setting (default is Gain 1 in O2k-Series G, gain 2 in previous Series). The maximum signal is 10 volt.  Above 9.99 V, the amplifier is in the saturation range, the apparent signal will remain constant at this value, and the slope will appear to be zero.  The raw signal can be modified by choosing an appropriate [[gain (O2 channel)|gain]] setting.
+|description=The '''raw signal''' of the polarographic oxygen sensor is the [[current]] ''I''<sub>el</sub> [µA], 1 µA = 10<sup>-6</sup> C·s<sup>-1</sup>, (DatLab 8) or the electric potential difference ([[voltage]]) [V], 1 V = 1 J·C<sup>-1</sup>, obtained after a current-to-voltage conversion in the O2k (DatLab 7 and previous versions).
+|info=[[Oxygen signal]]
-:::: Typical recorded voltages at air saturation are
+}}
-::::* at Gain 8: offscale ( “9.99V”)
+{{MitoPedia O2k and high-resolution respirometry
-::::* at Gain 4 : ca. 8 - 10 V (do not use at air saturation, especially if your lab is situated at or near sea level)
+|mitopedia O2k and high-resolution respirometry=DatLab, Oroboros QM
-::::* at Gain 2: ca. 4 - 5 V
+}}
-::::* at Gain 1: ca. 1 - 3 V
-:::: The raw signal observed during calibration at air saturation depends on experimental temperature, barometric pressure (lower values are obtained above sea level), and the sensitivity of the individual POS. The raw signal is converted to oxygen concentration [µM or nmol/ml] or partial oxygen pressure [kPa] by [[O2_calibration_-_DatLab| calibration of the POS]].  Each gain requires a separate calibration of the oxygen signal. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment), but also a new zero oxygen calibration.
-{{#set:Technical service=O2 sensor|Technical service=O2 signal}}