SUIT-022: Difference between revisions

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SUIT-022

Description

Reference: A: Determination of the respiration due to the alternative oxidase pathway »Versions

O2k-Application: O2

SUIT-category: ce

SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM

The SUIT-022 is designed to provide an estimation of the mitochondrial respiration in cells due to the alternative oxidase pathway, which is cyanide-and antimycin A-resistant. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome c pathway (see SUIT-023).

Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)

Specific SUIT protocols

SUIT-022 O2 ce D051

SUIT-022 O2 ce D051 for intact microalgal cells (ce)

Strengths and limitations

SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on cells on ROUTINE. To complement the study with the estimation of the contribution of the cytochrome c pathway to respiration, it must be carried out in parallel to SUIT-023.

+ This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.

+ Short duration of experiment

- Estimation of the linear coupling control is not done

- The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.

Compare SUIT protocols

References

MitoPedia concepts: MiP concept, SUIT protocol, Recommended

MitoPedia methods: Respirometry

Revision as of 11:09, 9 April 2019 (view source) Huete-Ortega Maria (talk \| contribs) No edit summary ← Older edit		Revision as of 11:09, 9 April 2019 (view source) Huete-Ortega Maria (talk \| contribs) No edit summary Newer edit →
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	::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM		::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM

	The SUIT-022 is designed to provide an estimation of the mitochondrial respiration in cells due to the [[alternative oxidase]] pathway, which is [[cyanide\| cyanide]]-and [[Antimycin_A\| antimycin A]]-resistant. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome c pathway (see [[SUIT-023]].		The SUIT-022 is designed to provide an estimation of the mitochondrial respiration in cells due to the [[alternative oxidase]] pathway, which is [[cyanide\| cyanide]]-and [[Antimycin_A\| antimycin A]]-resistant. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome c pathway (see [[SUIT-023]]).