Difference between revisions of "Salicylhydroxamic acid"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=SHAM | |abbr=SHAM | ||
|description='''Salicylhydroxamic acid''' (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the [[alternative oxidase]] (AOX). When AOX is blocked by SHAM, electrons are forced through the | |description='''Salicylhydroxamic acid''' (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the [[alternative oxidase]] (AOX). When AOX is blocked by SHAM, electrons are forced through the CIII-cytochrome ''c'' oxidase pathway, allowing observation of the operation of the CIII-CIV pathway without AOX activity. | ||
|info=[[Parrino_2000_J_Exp_Biol]] | |info=[[Parrino_2000_J_Exp_Biol]] | ||
}} | }} | ||
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:::'''Preparation of 20 mM stock solution (dissolved in DMSO)''': | :::'''Preparation of 20 mM stock solution (dissolved in DMSO)''': | ||
::::# Weigh 6.1 mg of SHAM. | ::::# Weigh 6.1 mg of SHAM. | ||
::::# Dissolve in 2 | ::::# Dissolve in 2 mL of DMSO. | ||
::::# Divide into 0.2 mL portions | ::::# Divide into 0.2 mL portions | ||
::::# Store at -20 °C. | ::::# Store at -20 °C. | ||
Revision as of 08:59, 15 June 2019
- high-resolution terminology - matching measurements at high-resolution
Salicylhydroxamic acid
Description
Salicylhydroxamic acid (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the alternative oxidase (AOX). When AOX is blocked by SHAM, electrons are forced through the CIII-cytochrome c oxidase pathway, allowing observation of the operation of the CIII-CIV pathway without AOX activity.
Abbreviation: SHAM
Reference: Parrino_2000_J_Exp_Biol
Application in HRR
- SHAM: Salicylhydroxamic acid (99%; HOC6H4CONHOH); Sigma S 607, 5 g, store at RT; FW = 153.14.
Caution: Irritant!
- Preparation of 20 mM stock solution (dissolved in DMSO):
- Weigh 6.1 mg of SHAM.
- Dissolve in 2 mL of DMSO.
- Divide into 0.2 mL portions
- Store at -20 °C.
- Preparation of 20 mM stock solution (dissolved in DMSO):
- O2k Manual Titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 100 µL
- Final conc. in 2 mL O2k chamber: 1 mM
MitoPedia topics:
Inhibitor
