Talk:MitoPedia: Respiratory states: Difference between revisions

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# How is a particular respiratory state obtained experimentally? Example: Addition of reduced carbon-substrate after ADP to obtain ‘[[State 3]]’, and evaluation of saturation of flux by experimental concentrations of ADP, P<sub>i</sub>, and O<sub>2</sub> to verify that OXPHOS capacity, state ''P'', rather than a substrate-limited flux is measured.
# How is a particular respiratory state obtained experimentally? Example: Addition of reduced carbon-substrate after ADP to obtain ‘[[State 3]]’, and evaluation of saturation of flux by experimental concentrations of ADP, P<sub>i</sub>, and O<sub>2</sub> to verify that OXPHOS capacity, state ''P'', rather than a substrate-limited flux is measured.


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Revision as of 13:23, 4 December 2015

Note on respiratory states

  1. Which question or concept provides the basis for studying a particular respiratory state? Example: OXPHOS capacity, state P.
  2. How is a particular respiratory state obtained experimentally? Example: Addition of reduced carbon-substrate after ADP to obtain ‘State 3’, and evaluation of saturation of flux by experimental concentrations of ADP, Pi, and O2 to verify that OXPHOS capacity, state P, rather than a substrate-limited flux is measured.
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