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Difference between revisions of "Talk:O2k-FluoRespirometer"

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'''Parameters:'''
'''Parameters:'''
* oxygen consumption
* Oxygen consumption
* ROS production
* ROS production
* ATP production  
* ATP production  
* Ca2+
* Ca<sup>2+</sup>
* pH
* pH
* mitochondrial membrane potential
* Mitochondrial membrane potential





Revision as of 09:42, 11 June 2019

                



Talk:O2k-FluoRespirometer

O2k-Catalogue

Description The Oroboros O2k-FluoRespirometer (O2k-Series H) - the experimental system complete for high-resolution respirometry (HRR), including fluorometry and the TIP2k, allowing simultaneous monitoring of oxygen consumption together with either ROS production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca2+ (CaG) or ATP production (MgG). An additional OroboPOS and MiR05-Kit are included in the delivery of this O2k-Package.

The O2k-FluoRespirometer supports all add-on O2k-Modules: O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module, enabling measurement of mt-membrane potential with ion sensitive electrode (ISE for TPP+ or TPMP+ ) or pH.


Parameters:

  • Oxygen consumption
  • ROS production
  • ATP production
  • Ca2+
  • pH
  • Mitochondrial membrane potential


The O2k is a sole source apparatus with no other instruments meeting its specifications.

Product ID 10002-02
Type O2k
Link Oroboros O2k, O2k-FluoRespirometer manual
Image
O2k-FluoRespirometer
O2k-FluoRespirometer
The O2k-FluoRespirometer[1] does not include, but fully supports add-on O2k-Modules: O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module.
  1. A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see here.

O2k-Series H: innovations

Publications in the MiPMap
About Oroboros
O2k-Series H: innovations


Oroboros (2017-07-11) Mitochondr Physiol Network

Abstract: Mészáros A, Krumschnabel G, Gradl P, Schwaninger H, Gnaiger E (2017) O2k-Series H: innovations. Mitochondr Physiol Network 22.09

O2k-Series H is the latest version of the Oroboros O2k for high-resolution respirometry and O2k-MultiSensor applications. The O2k-Series H sets a new standard in merging high-end technology and corporate social responsibility.


O2k-Network Lab: AT_Innsbruck_Oroboros


Features: integrated FluoRespirometry

  • The Fluorescence-control unit is integrated into the O2k-Main Unit. The new O2k-Window Frame is optimized for fixation of the Fluorescence-Sensors. The previous Window Tool, Front Fixation and Sensor-Guide are not required.
  • Smart Fluo-Sensors are pre-calibrated with sensor-specific memory and direct input into DatLab 7. O2k-Series A to G do not support the Smart Fluo-Sensors

New: DatLab 7

  • DatLab 7 with context-sensitive help.
  • DL-Protocols as real-time guides for titrations in DatLab 7, context-sensitive help. Further details » MitoPedia: DatLab.

Electronic innovations

  • High-resolution A-D converter: The new 24 bit analogue-digital converters provide a higher precision in measurement of O2, Amp-signals (amperometric fluorometry and nitric oxide measurements), and pX-signals (potentiometric measurements).
  • Core Relative Humidity and Core Relative Temperature: In addition to the built-in ambient temperature sensor, two new measurement channels are implemented to continuously monitor conditions within the electronic Main-Unit. Such data are especially important for quality control of experiments performed at high humidity and varying external temperature.
  • A 16 GB, industry-rated flash drive is integrated for storage of calibration files and other instrument-specific data. (This function will be available upon release of a later version of DatLab).
  • USB 2.0 Hi-Speed hub (4 external ports) for additional USB devices.
  • Three internal USB ports allow further upgrades and upwards compatibility with new developments.
  • Increased energy efficiency based on the new O2k-electronics design: power consumption is 24 W at 37 °C (80% reduction compared to Series D). Running costs are substantially lower as a result of our general concept for sustainable technology. The maximal power input is 120 W.
  • O2k net weight is reduced to 13.5 kg in comparison to 21 kg in Series D.






Previous O2k-Series D-G Product ID 10010-01


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