Difference between revisions of "Template:200 mM ADP MgG"
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(Created page with "::::Merck (former Calbiochem): 117105 (Adenosine 5ʹ-Diphosphate, Potassium Salt), store at -20°C. ::::# Weigh 1.0026 g, dilute in H<sub>2</sub>O ::::# Adjust pH to 6.9 with...") |
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::: Merck (former Calbiochem): 117105 (Adenosine 5ʹ-Diphosphate, Potassium Salt), store at -20°C. | |||
::::# Weigh 1.0026 g, dilute in H<sub>2</sub>O | ::::# Weigh 1.0026 g, dilute in H<sub>2</sub>O |
Latest revision as of 13:19, 3 September 2020
- Merck (former Calbiochem): 117105 (Adenosine 5ʹ-Diphosphate, Potassium Salt), store at -20°C.
- Weigh 1.0026 g, dilute in H2O
- Adjust pH to 6.9 with KOH, preferably on ice (with pHmeter calibrated on the same condition)
- Complete with H2O to 10 mL
- Aliquote (200 µL) and store at -20°C.
- Avoid thawing and re-freezing the aliquotes.
- The concentration can be corrected by measuring the absorbance at 260 nm and using an extinction coefficient factor of εM = 15400 M-1⋅cm-1