Step
|
State
|
Pathway
|
Q-junction
|
Comment - Events (E) and Marks (M)
|
ce1
|
ROUTINE
|
|
|
ce1
- ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
|
ce1DMSO
|
ROUTINE
|
|
|
ce1
- ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
|
(ce2Omy)
|
L(Omy)
|
|
|
ce1;ce1Snv;(ce2Omy)
- Non-phosphorylating resting state (LEAK state); LEAK-respiration, L(Omy), after blocking the ATP synthase with oligomycin.
- The addition of oligomycin is optional depending on the experimental question to resolve.
|
ce3U
|
E
|
|
|
ce1;ce1Snv;(ce2Omy);ce3U
|
ce4Rot
|
ROX
|
|
|
ce1;ce1Snv;(ce2Omy);ce3U;ce4Rot
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration.
|
ce5Ama
|
ROX
|
|
|
ce1;ce1Snv;(ce2Omy);ce3U;ce4Rot;ce5Ama
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration.
|