Template talk:Next oroboros ecosystem agenda: Difference between revisions
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| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] | | width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] | ||
|''' | |'''Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature? ''' <br> | ||
- Β»[[O2k-Peltier_Mitopedia#Block_temperature_does_not_increase_while_heating_with_full_power|Block temperature does not increase while heating with full power]]Β« and Β»[[DatLab error messages]] Β« | - Β»[[O2k-Peltier_Mitopedia#Block_temperature_does_not_increase_while_heating_with_full_power|Block temperature does not increase while heating with full power]]Β« and Β»[[DatLab error messages]] Β« | ||
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Revision as of 11:25, 7 May 2020
- Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
{| | logos | title in bold <br> - link |}
e.g.,
Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?
- Β»Instrumental backgroundΒ« and Β»TIP2k Filter PapersΒ«
- See the scheme:
Bioblast alert 2020
Prep for circular done here: Talk:Bioblast alert 2020
MitoPedia alert
Next
- Jun-14 Th
How can I measure ATP production in cells?
Respiration measurements with living cells provides information on parameters such as free ROUTINE activity. MitoPedia may help to clarify why defining it as ATP production is inappropriate
- Β»SUIT protocols to measure ATP productionΒ« Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green -
- Β»Magnesium GreenΒ«
- Jun-21 Th
As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O2 consumption and production
-Β»PhotoBiologyΒ«
O2k-Publications alert
Next
O2k-Open Support alert
Next
- While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
- Jun-08 Mo
Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature?
- Β»Block temperature does not increase while heating with full powerΒ« and Β»DatLab error messages Β«
Removed for correction
Do you perform the leaky chamber test after chamber assembly?
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- Β»Leaky chamber testΒ« and Β» https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assemblyΒ«
Do you perform a stirrer test during oxygen calibration?
In DatLab 7.4, instrumental DL-Protocols guide you automatically towards performing the stirrer test in the process of air calibration. It shows immediately, if your OroboPOS has a satisfactory response time. Further details in
- Β» MitoPedia: Β»Oxygen calibration - DatLab
Is the noise of the βO2 slope negβ acceptable?
If you are not certain that the noise of the βO2 slope negβ is within a suitable range or you want to confirm that your experimental data will not be affected, see:
- Β» MiPNet06.03 POS-calibration-SOP
O2k-Open Support alert circulars
O2k-Open Support alert circular 2020(01): in prep
New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
See how you can check that the two blue OroboPOS-Holders are intact.
- Β»O2k-OroboPOS-HolderΒ«
New Calibration parameters are transferred to experimental DatLab files
Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files.
- Β» Oxygen calibration - DatLabΒ«
Do you perform O2k-Chamber volume calibration?
The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module).
- Β»O2k-Chamber MitoPediaΒ«
Do you use the correct O2 solubility factor (FM) in the βO2 calibrationβ window in DatLab?
The O2 solubility factor varies according to the medium used and influences the air calibration.
- Β»Oxygen solubility factorΒ«
In preparation
For discussion
Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions: - Proven -80 Β°C solid stability for more than 3 years (3 years 7 months)
- Proven -20 Β°C solid stability for 1 year
- Proven DMSO stability at room temperature for 1 week
- Proven DMSO stability at -20 Β°C for 6 months (ongoing)
2021
- 2021-01-06 https://www.jstor.org/stable/1706346?seq=1