Difference between revisions of "Template talk:Next oroboros ecosystem agenda"
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| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]] | | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]] | ||
|'''Do you know how to prepare | |'''Do you know how to prepare permeabilized muscle fibers?''' | ||
The application of high-resolution respirometry in permeabilized muscle fibers provides a sensitive diagnostic tool for the analysis of mitochondrial (dys)function in small biopsy samples.<br> | The application of high-resolution respirometry in permeabilized muscle fibers provides a sensitive diagnostic tool for the analysis of mitochondrial (dys)function in small biopsy samples.<br> | ||
- »'''[https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be O2k-Videosupport: Fiber permeabilization]'''« <br> | - »'''[https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be O2k-Videosupport: Fiber permeabilization]'''« <br> | ||
- »'''[https://wiki.oroboros.at/index.php/Doerrier_2018_Methods_Mol_Biol Doerrier 2018 Methods Mol Biol]'''« <br> | - »'''[https://wiki.oroboros.at/index.php/Doerrier_2018_Methods_Mol_Biol Doerrier 2018 Methods Mol Biol]'''« <br> | ||
- communicated by »[[Doerrier Carolina]] and | - communicated by »[[Doerrier Carolina]] and [[Tindle-Solomon Lisa]]« | ||
|} | |} | ||
Revision as of 17:59, 11 February 2021
- Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
{| | logos | title in bold <br> - link |}
e.g.,
Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?
- »Instrumental background« and »TIP2k Filter Papers«
- See the scheme:
Bioblast alert 2021
Prep for circular:
MitoPedia alert
Next
- Nov-xx (in preparation)
What is the difference between flux control ratio and flux control efficiency used for internal normalization of respiration? Get to know a new term in cell ergometry!
Flux control ratios are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0. Flux control efficiency expresses the fractional control of respiration as a step change of oxygen flux or flow.
- »Flux control ratio« and »Flux control efficiency«
- » Komlodi Timea«, » Cecatto Cristiane« and » Gnaiger Erich«
Version 02.
What is the difference between flux control ratio and flux control efficiency used for internal normalization of respiration? Get to know a new term in cell ergometry!
Flux control ratio is ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the fractional control of respiration as a step change of oxygen flux or flow. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Flux control ratio« and »Flux control efficiency«
- » Komlodi Timea«, » Cecatto Cristiane« and » Gnaiger Erich«
Version 03.
Cell ergometry terminology- What is the difference between flux control ratio and flux control efficiency?
Flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the fractional control of respiration as a step change of oxygen flux or flow. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Flux control ratio« and »Flux control efficiency«
- » Komlodi Timea«, » Cecatto Cristiane« and » Gnaiger Erich«
Version 04
Cell ergometry terminology – What is the difference between flux control ratio and flux control efficiency?
Flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Flux control ratio« and »Flux control efficiency«
- » Komlodi Timea«, » Cecatto Cristiane« and » Gnaiger Erich«
O2k-Brief alert
Next
O2k-Publications alert
Author names of O2k-Publications/ Brief for the Agenda vs Bioblast
- 1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: Template alerts.
- Example: Oroboros Ecosystem Agenda example
- This means that we have to edit manually the author names within the O2k-Publications/ Brief alerts (i.e., Antunes Diana (from Bioblast) to Antunes D (to Agenda entries)) if they are long or copy from the gray box.
- 2.Bioblast: SOP: [1] – examples are available using both Surname and First name with long version (e.g., Antunes Diana). If in Bioblast the authors are with short surname in the links please update it to complete surname, guidelines are below.
- 1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: Template alerts.
Guidelines to update a reference
- When creating an O2k-Publication/Brief alert, it is the responsibility of the author to check if the reference is accordingly to the correct guidelines:
- Correct format for a person called “First Name Surname”:
- In the gray box should be “Surname FN” - From where references to be used as O2k-Publication/Brief alerts are copied from.
- In the links should be “ Surname First N”
- How to edit:
- A) Check if all authors with profiles in Bioblast (blue links) have their profiles with full name. If they have, proceed to next step (B). If not, you need to move it following the steps “How to move a profile” below.
- B) Click in “Edit with form”.
- C) The gray box is edited in “Reference” and the links are changed in “Author(s)”. Change it accordingly and click in “save page” at the end of the page.
- How to edit:
How to move a profile (instructions sent by Marija):
So let’s take for example my page: Beno M [2]
- As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps:
- 1. Go to e.g. “Beno M” on edit
- 2. Click on “Move”
- 3. Change the new title to the full name of the person. In my case it will be Beno M to Beno Marija
- As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps:
- 4. Now it should look like that:
- 5. Click on “Move” and leave all the ticked boxes
- 6.Done
- After that you can continue in step B.
- If you have any doubt you can contact Cristiane, Marija or Mario.
Next
---
O2k-Open Support alert
- While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
Next
What is the advantage of using flux control ratios (FCRs)?
FCRs provide information on coupling and substrate control of mitochondrial respiration independent of (i) mt-content in cells or tissues, (ii) purification of isolated mitochondrial preparations, and (iii) assay conditions for determination of tissue mass or mt-markers.
- »Flux control ratio«
Do you know how to prepare permeabilized muscle fibers? The application of high-resolution respirometry in permeabilized muscle fibers provides a sensitive diagnostic tool for the analysis of mitochondrial (dys)function in small biopsy samples.
- »O2k-Videosupport: Fiber permeabilization«
- »Doerrier 2018 Methods Mol Biol«
- communicated by »Doerrier Carolina and Tindle-Solomon Lisa«
Under discussion
Removed for correction
- Nov-30 Mo
Noisy oxygen signal? Check the OroboPOS and OroboPOS-Connector to clean electrical connections.
Contaminating grease, salt crystals and moisture can be removed with a dry paper cloth or a paper cloth moistened with water and then absolute ethanol.
- »Cleaning the electrical connections«
- communicated by »Schmitt Sabine« and »Komlodi Timea«
WGT recommended not to use water for cleaning. Erich introduced water for cleaning to remove salt crystals.
Do you perform the leaky chamber test after chamber assembly?
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«
O2k-Open Support alert circulars
In preparation
For discussion
Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions: - Proven -80 °C solid stability for more than 3 years (3 years 7 months)
- Proven -20 °C solid stability for 1 year
- Proven DMSO stability at room temperature for 1 week
- Proven DMSO stability at -20 °C for 6 months (ongoing)
2021
- 2021-01-06 https://www.jstor.org/stable/1706346?seq=1
Postponed events
- Pre-Conference O2k-Workshop »MiPNet25.11 IOC148 Riga LV « March 2021?
- »6th EU-Cardioprotection Meeting 2020 Riga LV« (Oct 7-9)- March 2021?
- »APS2020 Chicago US«
- Abstracts deadline 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology
- Notification to oral and poster presenters - »MitoEAGLE Summit Obergurgl 2020« (2020 Nov 04-07) and »MiPschool Obergurgl 2020« (2020 Nov 09-11)
- Bioenergetics Vienna 2020
- IOC Schroecken AT
- Registration and late Abstract Deadline - »MitoEAGLE Summit Obergurgl 2020« and »MiPschool Obergurgl 2020«