Template talk:Next oroboros ecosystem agenda
- Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
{| | logos | title in bold <br> - link |}
e.g.,
Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?
- »Instrumental background« and »TIP2k Filter Papers«
- See the scheme:
Bioblast alert 2020
Prep for circular done here: Talk:Bioblast alert 2020
MitoPedia alert
Next
- May-21 Th
As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O2 consumption and production
-»PhotoBiology«
- Jun-4 Th
Does your sample display a preference for glutamate & malate over pyruvate & malate to support NADH-linked respiration?
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to evaluate the contribution of different combinations of two NADH-linked respiration substrates.
-»SUIT-008« and »SUIT-014«
O2k-Brief alert
Next
- May-26 Tu
Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes
- from the O2k-Network » AT Innsbruck Gnaiger E « and » AT Innsbruck Oroboros «
- Schöpf Bernd, Weissensteiner Hansi, Schäfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, Sant’Anna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487. - »Bioblast link«
- from the O2k-Network » AT Innsbruck Gnaiger E « and » AT Innsbruck Oroboros «
O2k-Publications alert
Next
- May-17 Su World Hypertension Day
Mitochondrial DNA enhances the hypoxia-induced cardioprotective effect in hypertensive rats
- from the O2k-Network »CZ Prague Houstek J« and »CZ Prague Kalous M«
- Neckar J, Svatonova A, Weissova R, Drahota Z, Zajickova P, Brabcova I, Kolar D, Alanova P, Vasinova J, Silhavy J, Hlavackova M, Tauchmannova K, Milerova M, Ostadal B, Cervenka L, Zurmanova J, Kalous M, Novakova O, Novotny J, Pravenec M, Kolar F (2017) Selective replacement of mitochondrial DNA increases the cardioprotective effect of chronic continuous hypoxia in spontaneously hypertensive rats. Clin Sci (Lond) 131(9):865-881. »Bioblast link«
- May-22 Fr
F1Fo-ATP synthase regulates alternative oxidase in Trypanosoma brucei
- from the O2k-Network »BR Sao Paulo Silber AM«
- Alberto Luévano-Martínez L, Girard RMBM, Alencar MB, Silber AM (2020) ATP regulates the activity of an alternative oxidase in Trypanosoma brucei. FEBS Lett [Epub ahead of print]. »Bioblast link«
- Jun-2 Tu
Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers
- from the O2k-Network »AT Innsbruck Gnaiger E« , »CA Rimouski Blier PU« and »CA Edmonton Lemieux H«
- Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. »Bioblast link«
O2k-Open Support alert
Next
- While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
- Jun-08 Mo
Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature?
During air calibration, temperature of the chamber and Peltier power can be monitored under the layout '01 Calibration show Temp'. In case of an issue with the temperature control, an error message will appear. For more information, see: - »Block temperature does not increase while heating with full power« and »DatLab error messages «
Removed for correction
Do you perform the leaky chamber test after chamber assembly?
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«
Do you perform a stirrer test during oxygen calibration?
In DatLab 7.4, instrumental DL-Protocols guide you automatically towards performing the stirrer test in the process of air calibration. It shows immediately, if your OroboPOS has a satisfactory response time. Further details in
- » MitoPedia: »Oxygen calibration - DatLab
Is the noise of the ‘O2 slope neg’ acceptable?
If you are not certain that the noise of the ‘O2 slope neg’ is within a suitable range or you want to confirm that your experimental data will not be affected, see:
- » MiPNet06.03 POS-calibration-SOP
O2k-Open Support alert circulars
O2k-Open Support alert circular 2020(01): in prep
New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
See how you can check that the two blue OroboPOS-Holders are intact.
- »O2k-OroboPOS-Holder«
New Calibration parameters are transferred to experimental DatLab files
Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files.
- » Oxygen calibration - DatLab«
Do you perform O2k-Chamber volume calibration?
The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module).
- »O2k-Chamber MitoPedia«
Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
The O2 solubility factor varies according to the medium used and influences the air calibration.
- »Oxygen solubility factor«
In preparation
For discussion
Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions: - Proven -80 °C solid stability for more than 3 years (3 years 7 months)
- Proven -20 °C solid stability for 1 year
- Proven DMSO stability at room temperature for 1 week
- Proven DMSO stability at -20 °C for 6 months (ongoing)
2021
- 2021-01-06 https://www.jstor.org/stable/1706346?seq=1