Difference between revisions of "Vrbacky 2003 Physiol Res"
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{{Publication | {{Publication | ||
|title=Vrbacky M, Krijt J, Drahota Z, Melkova Z (2003) Inhibitory effects of Bcl-2 on mitochondrial respiration. Physiol. Res. 52: 545-554. | |title=Vrbacky M, Krijt J, Drahota Z, Melkova Z (2003) Inhibitory effects of Bcl-2 on mitochondrial respiration. Physiol. Res. 52: 545-554. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/14535829 PMID: 14535829] | |||
|authors=Vrbacky M, Krijt J, Drahota Z, Melkova Z | |authors=Vrbacky M, Krijt J, Drahota Z, Melkova Z | ||
|year=2003 | |year=2003 | ||
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|abstract=In contrast to the well-established anti-apoptotic effect of Bcl-2 protein, we have recently demonstrated that Bcl-2 overexpression by vaccinia virus causes apoptosis in BSC-40 cells, while it prevents apoptosis in HeLa G cells. Given the key role of mitochondria in the process of apoptosis, we focused on effects of Bcl-2 expression on mitochondrial energetics of these two cell lines. In this study we present data indicating that BSC-40 cells derive their ATP mainly from oxidative phosphorylation whereas HeLa G cells from glycolysis. More importantly, we show that in both cell lines, Bcl-2 inhibits mitochondrial respiration and causes a decrease of the ATP/ADP ratio. However, it appears that BSC-40 cells cannot sustain this decrease and die, while HeLa G cells survive, being adapted to the low ratio of ATP/ADP maintained by glycolysis. Based on this observation, we propose that the outcome of Bcl-2 expression is determined by the type of cellular ATP synthesis, namely that Bcl-2 causes apoptosis in cells relying on oxidative phosphorylation. | |abstract=In contrast to the well-established anti-apoptotic effect of Bcl-2 protein, we have recently demonstrated that Bcl-2 overexpression by vaccinia virus causes apoptosis in BSC-40 cells, while it prevents apoptosis in HeLa G cells. Given the key role of mitochondria in the process of apoptosis, we focused on effects of Bcl-2 expression on mitochondrial energetics of these two cell lines. In this study we present data indicating that BSC-40 cells derive their ATP mainly from oxidative phosphorylation whereas HeLa G cells from glycolysis. More importantly, we show that in both cell lines, Bcl-2 inhibits mitochondrial respiration and causes a decrease of the ATP/ADP ratio. However, it appears that BSC-40 cells cannot sustain this decrease and die, while HeLa G cells survive, being adapted to the low ratio of ATP/ADP maintained by glycolysis. Based on this observation, we propose that the outcome of Bcl-2 expression is determined by the type of cellular ATP synthesis, namely that Bcl-2 causes apoptosis in cells relying on oxidative phosphorylation. | ||
|keywords=Bcl-2, Apoptosis, ATP, Mitochondrial respiration | |keywords=Bcl-2, Apoptosis, ATP, Mitochondrial respiration | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|injuries=Cancer; Apoptosis; Cytochrome c | |instruments=Oxygraph-2k | ||
|injuries=Cancer; Apoptosis; Cytochrome c | |||
|tissues=Blood Cell; Suspension Culture | |tissues=Blood Cell; Suspension Culture | ||
| | |preparations=Permeabilized Cell or Tissue; Homogenate | ||
}} | }} | ||
Revision as of 11:53, 6 September 2011
| Vrbacky M, Krijt J, Drahota Z, Melkova Z (2003) Inhibitory effects of Bcl-2 on mitochondrial respiration. Physiol. Res. 52: 545-554. |
Vrbacky M, Krijt J, Drahota Z, Melkova Z (2003) Physiol. Res.
Abstract: In contrast to the well-established anti-apoptotic effect of Bcl-2 protein, we have recently demonstrated that Bcl-2 overexpression by vaccinia virus causes apoptosis in BSC-40 cells, while it prevents apoptosis in HeLa G cells. Given the key role of mitochondria in the process of apoptosis, we focused on effects of Bcl-2 expression on mitochondrial energetics of these two cell lines. In this study we present data indicating that BSC-40 cells derive their ATP mainly from oxidative phosphorylation whereas HeLa G cells from glycolysis. More importantly, we show that in both cell lines, Bcl-2 inhibits mitochondrial respiration and causes a decrease of the ATP/ADP ratio. However, it appears that BSC-40 cells cannot sustain this decrease and die, while HeLa G cells survive, being adapted to the low ratio of ATP/ADP maintained by glycolysis. Based on this observation, we propose that the outcome of Bcl-2 expression is determined by the type of cellular ATP synthesis, namely that Bcl-2 causes apoptosis in cells relying on oxidative phosphorylation. • Keywords: Bcl-2, Apoptosis, ATP, Mitochondrial respiration
Labels:
Stress:Cancer; Apoptosis; Cytochrome c"Cancer; Apoptosis; Cytochrome c" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property.
Tissue;cell: Blood Cell; Suspension Culture"Blood Cell; Suspension Culture" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property. Preparation: Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Oxygraph-2k
