https://wiki.oroboros.at/api.php?action=feedcontributions&user=Kranewitter+Sabine&feedformat=atomBioblast - User contributions [en]2024-03-29T06:35:51ZUser contributionsMediaWiki 1.36.1https://wiki.oroboros.at/index.php?title=Talk:Brunmair_2004_Diabetes&diff=229475Talk:Brunmair 2004 Diabetes2022-06-28T10:39:51Z<p>Kranewitter Sabine: Created page with "::::* Oroboros folder: 2004_4"</p>
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<div>::::* Oroboros folder: 2004_4</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Kuznetsov_2004_Am_J_Physiol_Heart_Circ_Physiol&diff=229474Talk:Kuznetsov 2004 Am J Physiol Heart Circ Physiol2022-06-28T10:38:33Z<p>Kranewitter Sabine: </p>
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<div>::::* Oroboros folder: 2008_1</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Gnaiger_2009_OCESHS&diff=229464Talk:Gnaiger 2009 OCESHS2022-06-28T10:30:07Z<p>Kranewitter Sabine: Created page with "::::* Oroboros folder: 2009_2"</p>
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<div>::::* Oroboros folder: 2009_2</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Gnaiger_2009_Int_J_Biochem_Cell_Biol&diff=229463Talk:Gnaiger 2009 Int J Biochem Cell Biol2022-06-28T10:29:31Z<p>Kranewitter Sabine: </p>
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<div>== Uncoupled respiration ==<br />
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::::* ".. apparent ET excess capacity (uncoupled respiration)": It will be helpful to distinguish physiological [[uncoupling]] and pathological [[dyscoupling]] from experimentally induced 'uncoupling' (using FCCP, DNP or other established uncouplers), by reference to the ET state as the [[noncoupled]] state of respiration [ [[MiPNet12.15]] ].<br />
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== Faculty of 1000 ==<br />
:::: This paper - "[[Boushel_2011_Mitochondrion|Muscle mitochondrial capacity exceeds maximal oxygen delivery in humans (2011)]]" - has been selected and evaluated by Max Gassmann, a Member of the [http://f1000.com Faculty of 1000]. More information for subscribers: http://f1000.com/8248956. <br />
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::: Evaluation details:<br />
:::: '''Sections''': Chemical Biology of the Cell,Integrative Physiology,Muscle & Connective Tissue<br />
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::: '''Comments''':<br />
:::: This work details our current understanding of oxygen transport and utilization while also contributing novel insight into mitochondrial physiology. Comparisons and references relating ex vivo measurements of mitochondrial respiration to various functional in vivo assessments of oxygen flux are greatly needed and this study provides just that. The study by Boushel et al. provides direct evidence advocating a mitochondrial respiratory reserve; a capacity for cellular respiration in skeletal muscle that can exceed the capacity for oxygen transport during maximal exercise. These results substantiate and extend upon findings of earlier, classic studies {1, 2}. The ability to examine mitochondrial function ex vivo via small intact tissue samples, such as permeabilized skeletal muscle fibers, has dramatically improved over the last decade {3} and is becoming commonplace in studies that vary greatly in topic. It is not, however, completely understood how representative or reflective these measurements are of in vivo mitochondrial function. This specific study found that ex vivo measurements of maximal oxidative phosphorylation (malate + octanoyl carnitine + glutamate + succinate + ADP-stimulated state 3 respiration) from a vastus lateralis tissue sample exceeded in vivo skeletal muscle oxygen consumption during lower-body maximal exercise consisting of 26% body mass (6.9 and 5.0 mmol O2 per kg & min, respectively) although respiratory capacities from deltoid muscle samples did not exceed oxygen consumption during upper-body maximal exercise, engaging only 8% body mass (4.3 and 4.7 mmol O2 per kg & min, respectively). The caveat being, however, that the respiratory capacities being compared, both of which were normalized to mass, were from different muscles. There is evidence demonstrating greater mitochondrial content per gram of skeletal muscle obtained from the vastus lateralis versus the deltoid {4}. Taking this into account, with the assumption that mitochondrial content of the deltoid is approximately 67% of that in the vastus lateralis {4}, differences in ex vivo respiratory capacities between lower- and upper-body muscle samples disappear. Moreover, while in vivo measurements of oxygen consumption in the leg during cycle ergometry remain lower than ex vivo respiratory measurements in the vastus lateralis, oxygen consumption during maximal arm crank ergometry may actually exceed OXPHOS capacity (maximal State 3 respiration) in the deltoid. An earlier study examining lower limb oxygen consumption during knee extensor exercise, involving only 3% body mass, reported in vivo oxygen consumption of approximately 13.3 mmol O2 per kg & min {2}. This dramatically exceeds the current study's reported maximal State 3 respiration measured from vastus lateralis samples, which would only account for 52% of in vivo oxygen consumption.<br />
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:::: These measurements correspond with previous in vitro measurements of isolated mitochondrial respiration that accounted for, at most, 60% of measured in vivo oxygen consumption {2}. Electron transport capacity, or maximal non-coupled respiration, does exceed maximal oxidative phosphorylation capacity in human skeletal muscle {5}; however, the physiologic relevance of this phenomenon is unknown. Are in vivo measurements of oxygen consumption above those of ex vivo maximal State 3 respiration a result of mitochondrial uncoupling? Or rather, an aberration of ex vivo respiratory measurements? Future studies should compare in vivo oxygen consumption during maximal exercise engaging only a small muscle mass to ex vivo measurements of maximal, non-coupled, electron transport capacity to help clarify these questions.<br />
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::: '''References''':<br />
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:::: {1} Andersen, Saltin (1985) J. Physiol. 366: 233-249 [PMID:4057091].<br />
:::: {2} Rasmussen et al (2001) Am. J. Physiol. Endocrinol. Metab. 280: E301-307 [PMID:11158934].<br />
:::: {3} [[Kuznetsov_2008_Nat Protoc|Kuznetsov et al (2008) Nat. Protoc. 3: 965-976]] [PMID:18536644].<br />
:::: {4} [[Larsen_2009_Diabetologia|Larsen et al. (2009) Diabetologia 52: 1400-1408]] [PMID:19396425].<br />
:::: {5} [[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger E (2009) Int. J. Biochem. Cell Biol. 41: 1837-1845]] [PMID:19467914].</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Jacobs_2011_J_Appl_Physiol&diff=228440Talk:Jacobs 2011 J Appl Physiol2022-06-08T10:17:41Z<p>Kranewitter Sabine: </p>
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== Bioblast Alert ==<br />
: 2011-10-26 (252 visits)<br />
* '''How to separate changes in mitochondrial quality and quantity'''. Does training induce mild uncoupling? Are sedentary humans healthy controls? - ''See'' [[Pesta_2011_AJP]]</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Gnaiger_2019_MitoPathways&diff=228436Talk:Gnaiger 2019 MitoPathways2022-06-08T10:00:07Z<p>Kranewitter Sabine: Removed redirect to Talk:Gnaiger 2020 MitoPathways</p>
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#REDIRECT [[Talk:Gnaiger 2020 MitoPathways]]</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Iyer_2012_Hum_Gene_Ther&diff=227066Talk:Iyer 2012 Hum Gene Ther2022-05-11T10:01:45Z<p>Kranewitter Sabine: </p>
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<div>::::* Oroboros folder: 2012_5</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Robach_2012_Br_J_Sports_Med&diff=227064Talk:Robach 2012 Br J Sports Med2022-05-11T09:37:01Z<p>Kranewitter Sabine: </p>
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#REDIRECT [[Talk:Gnaiger 2020 BEC MitoPathways]]</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Chicco_2014_J_Exp_Biol&diff=226566Talk:Chicco 2014 J Exp Biol2022-04-20T09:21:28Z<p>Kranewitter Sabine: Created page with "::::* Oroboros folder: 2014_5"</p>
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<div>::::* Oroboros folder: 2014_5</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Larsen_2014_Anal_Biochem&diff=226565Talk:Larsen 2014 Anal Biochem2022-04-20T09:16:06Z<p>Kranewitter Sabine: Created page with "::::* Oroboros folder: 2014_4"</p>
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<div>::::* Oroboros folder: 2014_4</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Boushel_2014_Acta_Physiol_(Oxf)&diff=226564Talk:Boushel 2014 Acta Physiol (Oxf)2022-04-20T09:15:20Z<p>Kranewitter Sabine: Created page with "::::* Oroboros folder: 2014_3"</p>
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<div>::::* Oroboros folder: 2014_3</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Khan_2014_J_Comp_Physiol&diff=226563Talk:Khan 2014 J Comp Physiol2022-04-20T09:14:24Z<p>Kranewitter Sabine: Created page with "::::* Oroboros folder: 2014_2"</p>
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<div>::::* Oroboros folder: 2014_2</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Blatzer_2015_Antioxid_Redox_Signal&diff=226562Talk:Blatzer 2015 Antioxid Redox Signal2022-04-20T09:13:22Z<p>Kranewitter Sabine: Created page with "::::* Oroboros folder: 2015_8"</p>
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<div>::::* Oroboros folder: 2015_8</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Dueregger_2015_PLoS_One&diff=226561Talk:Dueregger 2015 PLoS One2022-04-20T09:12:45Z<p>Kranewitter Sabine: Created page with "::::* Oroboros folder: 2015_7"</p>
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<div>::::* Oroboros folder: 2015_7</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Burtscher_2015_Mitochondrion&diff=226560Talk:Burtscher 2015 Mitochondrion2022-04-20T09:12:09Z<p>Kranewitter Sabine: </p>
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[[Image:BB-Bioblast.jpg|left|40px|link=Bioblast:About|Bioblast wiki]]<br />
== Popular Bioblast page ==<br />
::: [[Burtscher 2015 Mitochondrion]] has been accessed more than <br />
::::* 5,000 times (2019-12-16)</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Oexle_1999_Biochim_Biophys_Acta&diff=225821Talk:Oexle 1999 Biochim Biophys Acta2022-03-30T08:52:36Z<p>Kranewitter Sabine: Created page with "::::* Oroboros folder: 1999_1"</p>
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<div>::::* Oroboros folder: 1999_1</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Lassnig_1998_BTK&diff=225820Talk:Lassnig 1998 BTK2022-03-30T08:50:31Z<p>Kranewitter Sabine: Created page with "::::* Oroboros folder: 1998_6"</p>
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<div>::::* Oroboros folder: 1998_6</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Kuznetsov_1998_BTK&diff=225819Talk:Kuznetsov 1998 BTK2022-03-30T08:49:48Z<p>Kranewitter Sabine: </p>
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<div>::::* Oroboros folder: 1998_5<br />
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[[Image:BB-Bioblast.jpg|left|40px|link=Bioblast:About|Bioblast wiki]]<br />
== Popular Bioblast page ==<br />
::: [[Kuznetsov 1998 BTK]] has been accessed more than <br />
::::* 5,000 times (2019-12-16)</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Gnaiger_1998_BTK-HRR&diff=225818Talk:Gnaiger 1998 BTK-HRR2022-03-30T08:48:31Z<p>Kranewitter Sabine: </p>
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<div>::::* Oroboros folder: 1998_4<br />
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[[Image:BB-Bioblast.jpg|left|40px|link=Bioblast:About|Bioblast wiki]]<br />
== Popular Bioblast page ==<br />
::: [[Gnaiger 1998 BTK-HRR]] has been accessed more than <br />
::::* 5,000 times (2019-12-16)</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Gnaiger_1998_BTK-COX&diff=225817Talk:Gnaiger 1998 BTK-COX2022-03-30T08:47:39Z<p>Kranewitter Sabine: Created page with "::::* Oroboros folder: 1998_3"</p>
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<div>::::* Oroboros folder: 1998_3</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Gnaiger_1998_Biochim_Biophys_Acta&diff=225816Talk:Gnaiger 1998 Biochim Biophys Acta2022-03-30T08:46:29Z<p>Kranewitter Sabine: </p>
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<div>::::* Oroboros folder: 1998_2<br />
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[[Image:BB-Bioblast.jpg|left|40px|link=Bioblast:About|Bioblast wiki]]<br />
== Popular Bioblast page ==<br />
::: [[Gnaiger 1998 Biochim Biophys Acta]] has been accessed more than <br />
::::* 5,000 times (2019-12-16)</div>Kranewitter Sabinehttps://wiki.oroboros.at/index.php?title=Talk:Gnaiger_1998_J_Exp_Biol&diff=225815Talk:Gnaiger 1998 J Exp Biol2022-03-30T08:45:32Z<p>Kranewitter Sabine: </p>
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<div>::::* Oroboros folder: 1998_1<br />
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[[Image:BB-Bioblast.jpg|left|40px|link=Bioblast:About|Bioblast wiki]]<br />
== Popular Bioblast page ==<br />
::: [[Gnaiger 1998 J Exp Biol]] has been accessed more than <br />
::::* 5,000 times (2017-01-06)</div>Kranewitter Sabine