From Bioblast
- high-resolution terminology - matching measurements at high-resolution
2-Deoxyglucose
Description
2-Deoxyglucose, also known as 2-deoxy-D-glucose is a glucose derivative that has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase.
Abbreviation: 2-DG
Reference: Proton_flux, ISE pH-Module
Application in HRR
- 2-DG: 2-deoxy-D-glucose (C6H1205); Sigma D6134, 1 g or 5 g, store at 2-8 °C; M = 164.16 g·mol-1
- Preparation of 2 M stock solution (dissolved in H2O):
- weigh 1 g of 2-deoxy-D-glucose
- dissolve with 3.0458 mL H2O in a 10 mL-glass beaker
- »Oroboros manual titrations: OroM2.4 SUITable
- Titration volume: 25 µL using a 50 µL syringe
- Final conc. in 2-mL chamber: 25 mM
MitoPedia topics:
Inhibitor
