Aasum 2017 Abstract MITOEAGLE Barcelona
Mitochondrial function in the diabetic heart – effect of an acute fat-load.
Event: MitoEAGLE Barcelona 2017
Altered myocardial metabolism and cardiac inefficiency are hallmarks of the diabetic heart, and may play a central role in the pathogenesis of diabetes related cardiac dysfunction (diabetic cardiomyopathy). Although high levels of fatty acids has been demonstrated to have adverse effects in the normal heart, its effect in the obese/diabetic heart is less clear. In the present study we have examined how an acute fat-load on the heart diet-induced obese (DIO) mice (20 week on a high-fat diet) and age-matched controls (CON) will influence mitochondrial respiration. We found reduced OXPHOS respiration in isolated mitochondrial from DIO as compared to CON hearts. By subjecting CON hearts to a high fat-load (elevated levels of fatty acids prior to mitochondrial isolation), OXPHOS respiration and RCR (respiratory coupling ratio) were reduced. These changes were not observed in mitochondria from DIO hearts, which may suggest that in diabetes, the heart undergoes adaptation to chronic exposure of elevated circulating fatty acids, which protect these hearts from the adverse effects of an acute fat-load.
Labels: MiParea: Exercise physiology;nutrition;life style Pathology: Diabetes, Obesity
Organism: Mouse Tissue;cell: Heart Preparation: Isolated mitochondria
Coupling state: OXPHOS
HRR: Oxygraph-2k Event: A2
- Cardiovascular Research Group, Dept Medical Biol, Fac Health Sciences, Univ Tromsø - The Arctic Univ Norway, Norway