Abou-Hamdan 2015 Methods Enzymol
|Abou-Hamdan A, Guedouari-Bounihi H, Lenoir V, Andriamihaja M, Blachier F, Bouillaud F (2015) Oxidation of H2S in mammalian cells and mitochondria. Methods Enzymol 554:201-28.|
Abstract: Hydrogen sulfide (H2S) is the third gasotransmitter described in mammals. These gasotransmitters (H2S, CO, and NO) are small molecules able to diffuse freely across membranes and thus susceptible to reach easily intracellular targets, one of which is the respiratory enzyme cytochrome oxidase subject to complete inhibition by low micromolar concentrations of these gases. However in contrast to NO or CO, H2S can be metabolized by a sulfide quinone reductase feeding the mitochondrial respiratory chain with the hydrogen atoms of sulfide. Sulfide is thus a two-sided molecule: substrate or poison according to the concentration. The aim of this chapter is to present a mean to monitor sulfide oxidation by isolated mitochondria or cells and to summarize how the properties of this amazing couple (mitochondria and sulfide) translate into practical and conceptual consequences.
• O2k-Network Lab: FR Paris Bouillaud F
Labels: MiParea: Respiration, Instruments;methods
Organism: Human, Pig, Mouse, Rat Tissue;cell: Heart, Nervous system, Liver, Kidney, Genital, CHO, Neuroblastoma, Fibroblast Preparation: Intact cells, Homogenate, Isolated mitochondria
Regulation: Coupling efficiency;uncoupling Coupling state: OXPHOS Pathway: N, S, CIV HRR: Oxygraph-2k, TIP2k