Bhattarai 2015 Abstract MiPschool Greenville 2015
|Exercise improves whole-body and skeletal muscle oxidative capacity in burned children.|
Event: MiPschool Greenville 2015
Burn victims undergo prolonged immobilization and become profoundly cachectic after injury [1,2]. Loss of muscle mass and function leads to reduced quality of life in burn survivors. Rehabilitative exercise training (RET) has been shown to increase muscle mass and strength while also increasing peak oxygen uptake (peak VO2) in burn survivors . However, the mechanisms responsible for these improvements in physiological function are not fully understood. Here, we studied the impact of chronic RET on whole body (peak VO2) and skeletal muscle (OXPHOS) oxidative capacity.
Fourteen children (7-17 years) with burns covering ≥30% of their total body surface area were studied. After discharge from hospital, patients performed a 6-week supervised RET program. RET consisted of both resistive and aerobic exercise performed at least 3-times weekly on non-consecutive days. Peak VO2 and muscle OXPHOS capacity were determined before and after the RET program. Peak VO2 was determined using a modified Bruce protocol treadmill test. Muscle OXPHOS capacity was determined in permeabilized muscle fibers by high-resolution respirometry.
Peak VO2 increased significantly after RET (33.0±2.2 vs. 28.1± 1.7 ml.min-1.kg-1; P<0.001). Muscle OXPHOS capacity increased by 43% after RET (46.7±4.5 vs. 32.5±4.9 pmol.s-1.mg-1, P<0.05). In agreement with the published literature, RET increases aerobic exercise capacity in burn survivors. Here, we show that this is accompanied by an increase in skeletal muscle OXPHOS capacity. We suggest that RET improves whole body physiological function in burn survivors at least part, by improving skeletal muscle OXPHOS capacity.
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Patients
Organism: Human Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue
Coupling state: OXPHOS
HRR: Oxygraph-2k Event: Poster
1-Metabolism Unit, Shriners Hospitals for Children; 2-Dept Surgery, Univ Texas Medical Branch, Galveston, Texas, USA. - [email protected]
References and acknowledgements
- Porter C, Hardee JP, Herndon DN, Suman OE (2015) The role of exercise in the rehabilitation of patients with severe burns. Exerc Sport Sci Rev 43.1:34-40.
- Porter C, Herndon DN, Borsheim E, Chao T, Reidy PT, Borack MS, Rasmussen BB, Chondronikola M, Saraf MK and Sidossis LS (2014) Uncoupled skeletal muscle mitochondria contribute to hypermetabolism in severely burned adults. Am J Physiol Endocrinol Metab 307.5:E462-7. - »Bioblast link«