Blebbistatin

high-resolution terminology - matching measurements at high-resolution

Blebbistatin

Description

Blebbistatin is a widely used muscle and non-muscle myosin II-specific inhibitor that block contractile activity. Blebbistatin shows selectivity and high affinity for multiple class II myosins. Blebbistatin is commonly employed in respirometric experiments with permeabilized muscle fibers (pfi). Permeabilized muscle fibers are sensitive to low oxygen supply due to diffusion restrictions that limit mitochondrial respiration at the core of the fiber bundle. Therefore, hyperoxic conditions are required to counteract this limitation. Further studies have shown that the addition of blebbistatin in the respiration medium prevents fiber contraction, reduces the oxygen sensitivity and allows the study of ADP kinetics in pfi at normoxic oxygen levels. However, other studies described that the presence of blebbistatin does not prevent the oxygen dependence in pfi. Moreover, several limitations of blebbistatin i.e. low solubility in water, cytotoxicity and phototoxicity have been described.

Abbreviation: Bleb

Reference: Perry 2011 Biochem J

References: Blebbistatin

	Year	Reference	Organism	Tissue;cell
Doerrier 2024 Free Radic Biol Med	2024	Doerrier C, Gama-Perez P, Pesta D, Distefano G, Soendergaard SD, Maise Chroeis K, Gonzalez-Franquesa A, Goodpaster BH, Prats C, Sales-Pardo M, Guimera R, Coen PM, Gnaiger E, Larsen S, Garcia-Roves PM (2024) Harmonization of experimental procedures to assess mitochondrial respiration in human permeabilized skeletal muscle fibers. Free Radic Biol Med 223:384-97. https://doi.org/10.1016/j.freeradbiomed.2024.07.039	Human	Skeletal muscle
MitoEAGLE WG2 Innsbruck AT	2020-03-09	Innsbruck AT, 2020 Mar 9-13. WG 2 on human skeletal muscle fibers retreat - COST Action MitoEAGLE.
MitoEAGLE Obergurgl 2019-01-31	2019-01-31	Obergurgl AT, 2019 Jan 31- Feb 03. WG 1 and 2 human skeletal muscle workshop and retreat - COST Action MitoEAGLE.
MitoEAGLE Copenhagen 2018	2018-08-06	Copenhagen DK, 2018 Aug 06-10. WG 1 and 2 human skeletal muscle workshop and retreat - COST Action MitoEAGLE.
Perry 2011 Biochem J	2011	Perry CG, Kane DA, Lin CT, Kozy R, Cathey BL, Lark DS, Kane CL, Brophy PM, Gavin TP, Anderson EJ, Neufer PD (2011) Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal muscle. Biochem J 437:215-22.	Human Rat	Skeletal muscle

	Year	Reference	Organism	Tissue;cell
Doerrier 2019 MiP2019	2019	Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers.	Human	Skeletal muscle
Doerrier 2018 MiP2018a	2018	Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers.	Mouse	Skeletal muscle
Doerrier 2018 MiP2018c	2018	Inter-laboratory study on human permeabilized myofibers: effects of fiber preparation, respiration buffer, and blebbistatin on mitochondrial respiratory capacity and oxygen limitation.	Human	Skeletal muscle
Doerrier 2017 MiP2017 WG2	2017	Upper limit of OXPHOS capacity in permeabilized myofibers: effect of oxygen, ADP and blebbistatin in MiR05Cr and Buffer Z.	Mouse	Skeletal muscle
Bezuidenhout 2016a Abstract MitoFit Science Camp 2016	2016	Comparison of oxygen dependence of respiration in permeabilized mouse skeletal muscle fibers in two respiration media, MiR06Cr and Buffer Z containing Ctl, Cr and Blebbistatin.	Mouse	Skeletal muscle

Kovács M, Tóth J, Hetényi C, Málnási-Csizmadia A, Sellers JR (2004) Mechanism of blebbistatin inhibition of myosin II. J Biol Chem 279(34):35557-63. - https://www.ncbi.nlm.nih.gov/pubmed/15205456
Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25 - Scandurra 2010 Adv Exp Med Biol
Várkuti BH, Képiró M, Horváth IÁ, Végner L, Ráti S, Zsigmond Á, Hegyi G, Lenkei Z, Varga M, Málnási-Csizmadia A (2016) A highly soluble, non-phototoxic, non-fluorescent blebbistatin derivative. Sci Rep 6:26141 - https://www.ncbi.nlm.nih.gov/pubmed/27241904

MitoPedia concepts: MiP concept

MitoPedia topics: Inhibitor