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Borutaite 2015 Abstract MiPschool Cape Town 2015

From Bioblast
Mitochondria and regulation of cell death during ischemia.


Borutaite V (2015)

Event: MiPschool Cape Town 2015

Opening of mitochondrial permeability transition pore (MPTP) is considered as one of the main determinants in ischemia-reperfusion induced myocardial injury. The involvement of MPTP in brain ischemia induced damage is less clear. MPTP causes permeabilization of mitochondrial membranes leading to the release of cytochrome c from mitochondria, mitochondrial dysfunction, caspase activation and apoptotic or necrotic death of cardiac or neuronal cells depending on cellular resources of ATP. In recent years various strategies aiming at pharmacological inhibition of MPTP during ischemia or reperfusion have been proposed, though the molecular mechanisms of regulation of MPTP are still unclear. The molecular composition of MPTP is also unresolved question. We have recently shown that low levels of NO may activate signalling pathways in cardiac and neuronal cells that involve protein kinases C and G which, in turn, may increase resistance of mitochondria to Ca2+- and ischemia-induced opening of MPTP. Analysis of phosphoproteome of mitochondria isolated from hearts treated with NO revealed several mitochondrial proteins, phosphorylation of which is affected by this treatment and which may be involved in MPTP formation. In this lecture, we will discuss current knowledge on structure of MPTP, experimental approaches to investigate MPTP functions and possible role of MPTP in ischemic heart and brain damage.

β€’ O2k-Network Lab: LT Kaunas Borutaite V


Stress:Ischemia-reperfusion  Organism: Human  Tissue;cell: Heart  Preparation: Isolated mitochondria 


Inst Neurosc, Lithuanian Univ Health Sc, Kaunas, Lithuania. - [email protected]

Suggested reading

  1. Borutaite V, Toleikis A, Brown GC (2013) In the eye of the storm: mitochondrial damage during heart and brain ischaemia. FEBS J 280:4999-5014.
  2. Borutaite V (2010) Mitochondria as decision-makers in cell death. Eviron Mol Mutagen 51:406-16.
  3. Ong SB, Samangouei P, Kalkhoran SB, Hausenloy DJ (2015) The mitochondrial ermeability transition pore and its role in myocardial ischemia reperfusion injury. J Mol Cell Cardiol 78:23-34.