Burtscher J 2012 Abstract Bioblast
|Burtscher J, Eigentler A, Gnaiger E, Schwarzer C (2012) Mitochondrial function in the mouse hippocampus. Mitochondr Physiol Network 17.12.|
Event: Bioblast 2012
Epilepsy is one of the most common neurological diseases featuring a prevalence of 1-2%. A high percentage of patients is refractory to antiepileptic medication, especially in mesial temporal lobe epilepsy (mTLE). Epilepsy is characterized by seizures, in which a lot of glutamate is released leading to excitotoxicity and neuronal loss. Seizure related alterations in neurons are often associated with damaged mitochondria and with impaired functions of distinct complexes of the electron transport chain in human patients and animal models. However, mitochondrial alterations during the development of epilepsy (epileptogenesis) are not well characterized and it is not yet known, whether mitochondrial alterations are cause or consequence of epileptogenesis. Answers to these questions are important to learn more about the neurochemical processes underlying epileptogenesis and to assess implications on the development of antiepilept(ogen)ic medication. Therefore, we are in the process of developing protocols to analyze different mitochondrial parameters using the Oxygraph-2K (Oroboros Instruments, Innsbruck) in hippocampal tissue - which is strongly affected in mTLE - of mice. We apply the kainic acid model of TLE in mice. Injection of kainic acid into the hippocampal CA1 region results in Status epilepticus, a subsequent silent phase and ultimately recurrent seizures. We want to study the activities of electron transport chain (ETC) complexes I, II and IV across different time points of these phases of epileptogenesis.
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• Keywords: Epilepsy, Kainic Acid
• O2k-Network Lab: AT Innsbruck Oroboros
Stress:Mitochondrial disease Organism: Mouse Tissue;cell: Nervous system Preparation: Homogenate Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex IV;cytochrome c oxidase
Pathway: N, S, CIV HRR: Oxygraph-2k
Burtscher Johannes (1), Eigentler Andrea (2), Gnaiger Erich (2, 3), Christoph Schwarzer (1)
(1) Institute of Pharmacology; Innsbruck, Austria; Email: [email protected]
(2) Medical University of Innsbruck, Department of Visceral, Transplant and Thoracic Surgery, D. Swarovski Research Laboratory, Innsbruck, Austria
(3) Oroboros Instruments Corp, High-Resolution Respirometry, Innsbruck, Austria