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Calderon-Dominguez 2017 Adv Sci (Weinh)

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Publications in the MiPMap
Calderon-Dominguez M, Alcalá M, Sebastián D, Zorzano A, Viana M, Serra D, Herrero L (2017) Brown adipose tissue bioenergetics: a new methodological approach. Adv Sci (Weinh) 4:1600274.

» PMID: 28435771 Open Access

Calderon-Dominguez M, Alcala M, Sebastian D, Zorzano A, Viana M, Serra D, Herrero L (2017) Adv Sci (Weinh)

Abstract: The rediscovery of brown adipose tissue (BAT) in humans and its capacity to oxidize fat and dissipate energy as heat has put the spotlight on its potential as a therapeutic target in the treatment of several metabolic conditions including obesity and diabetes. To date the measurement of bioenergetics parameters has required the use of cultured cells or extracted mitochondria with the corresponding loss of information in the tissue context. Herein, we present a method to quantify mitochondrial bioenergetics directly in BAT. Based on XF Seahorse Technology, we assessed the appropriate weight of the explants, the exact concentration of each inhibitor in the reaction, and the specific incubation time to optimize bioenergetics measurements. Our results show that BAT basal oxygen consumption is mostly due to proton leak. In addition, BAT presents higher basal oxygen consumption than white adipose tissue and a positive response to b-adrenergic stimulation. Considering the whole tissue and not just subcellular populations is a direct approach that provides a realistic view of physiological respiration. In addition, it can be adapted to analyze the effect of potential activators of thermogenesis, or to assess the use of fatty acids or glucose as a source of energy. Keywords: Bioenergetics, Brown adipose tissue, Mitochondria, Oxygen consumption rate Bioblast editor: Kandolf G O2k-Network Lab: ES Barcelona Zorzano A

Labels: MiParea: Respiration 

Organism: Human  Tissue;cell: Fat