From Bioblast
Description
Carboxyatractyloside CAT is a highly selective and potent inhibitor of the adenine nucleotide translocator (ANT). CAT stabilizes the nucleoside binding site of ANT on the cytoplasmic (positive) side of the inner membrane and blocks the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the c conformation of ANT leading to permeability transition pore (PTP) opening, loss of mitochondrial membrane potential, and apoptosis.
Abbreviation: CAT
Reference: MiPNet03.02, MiPNet09.12 Manual titrations of SUIT chemicals
Application in HRR
- CAT: Carboxyatractyloside (carboxyatractyloside potassium salt from Xanthium sibiricum; C31H46O18S2*xK+), Sigma-Aldrich: C4992, store at -20 Β°C, CAS: 77228-71-8, M = 802.99 gΒ·mol-1
- Hazard statements: H301, H311, H331; toxic if swallowed, toxic in contact with skin, toxic if inhaled
- CAT: Carboxyatractyloside (carboxyatractyloside potassium salt from Xanthium sibiricum; C31H46O18S2*xK+), Sigma-Aldrich: C4992, store at -20 Β°C, CAS: 77228-71-8, M = 802.99 gΒ·mol-1
- Alternative source: Merck 216201-5MG (Calbiochem, Carboxyatractyloside potassium salt from Xanthium sibiricum, C31H43O18S2K3*3H2O), 5 mg, store at -20 Β°C, M = 939.1 gΒ·mol-1.
- Preparation of 2 mM stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- For a vial with 2 mg of carboxyatractyloside: dissolve in 1065 Β΅L H2O.
- Divide into 100 Β΅L aliquots.
- Store at -20 Β°C.
- Β» Oroboros manual titrations MiPNet09.12 Manual titrations of SUIT chemicals
- Titration volume (2-mL O2k-chamber): 1-5 Β΅L using a 10 Β΅L Hamilton syringe.
- Experimental concentration: 1-5 Β΅M.
- Preparation of 0.5 mM solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Take 50 Β΅L of the 2 mM stock solution.
- Add 150 Β΅L H2O.
- Β» Oroboros manual titrations MiPNet09.12 Manual titrations of SUIT chemicals
- Titration volume (0.5-mL O2k-chamber): 1-5 Β΅L using a 10 Β΅L Hamilton syringe.
- Experimental concentration: 1-5 Β΅M.
- Comment: CAT is considered to be much more specific than Atractyloside (Atr), and the effective concentration required is much much lower (Β΅M). With Atr, a very high concentration (mM) is required for full inhibition. CAT appears to be little permeable to intact cells and can thus only be applied in permeabilized cells and tissues or in isolated mitochondria (Ruas 2016 PLOS_ONE).
Β» Talk:Carboxyatractyloside
MitoPedia topics:
Inhibitor