Coen 2014 Abstract MiP2014

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Exercise improves insulin sensitivity and muscle mitochondrial respiration following Roux-en-Y gastric bypass surgery.
Link:
Coen P
Mitochondr Physiol Network 19.13 - MiP2014

Coen P, DiStefano G, Tanner C, Helbling NL, Dubis G, Toledo F, Xie H, Eid G, Houmard J, Goodpaster BH (2014)

Event: MiP2014

The impact of exercise on insulin sensitivity and mitochondrial respiration following Roux-en-Y Gastric Bypass (RYGB) surgery is unknown. Our aim was to examine the effects of RYGB surgery-induced weight loss, both with and without aerobic exercise, on insulin sensitivity and mitochondrial respiration.

One to three months following RYGB, participants (N=128) were randomized to six-months of either moderate structured exercise (EX) or lifestyle education control (CON). Insulin sensitivity (SI) was determined by intravenous glucose tolerance test (IVGTT). Biopsies of the vastus lateralis were obtained, before and after the six-month interventions. Mitochondrial respiration was measured using two SUIT protocols in permeabilized muscle fiber bundles. Data were analyzed using a per-protocol (PP) approach to assess the efficacy of exercise performed (>120 min/week).

One hundred participants completed the a priori defined intervention protocols: (EX, N=44; CON, N=56). Weight loss was similar for both groups (Post CON vs. Post EX; 84.2±21.3 vs. 84.3±17.2 kg, P=0.23). Cardiorespiratory fitness was improved in EX but not in CON. EX provided an additive improvement in SI (Post CON vs. Post EX; 3.80±2.1 vs. 4.86±2.6, P=0.002) and only EX improved Complex I (CI; Pre EX vs. Post EX; 141±65 vs. 195±71 pmol∙s-1∙mg-1, P=0.018) and Complex I&II (CIⅈ Post CON vs. Post EX; 264±82 vs. 323±102 pmol∙s-1∙mg-1, P=0.02) supported OXPHOS capacity (state P). Interestingly, there was a robust time effect for increased phosphorylation system control ratio (P/E) (P=0.006) and increase in the CI/CI&IIP substrate control ratio (P=0.007), suggesting surgery-induced weight loss specific effects on mitochondrial respiration. Aerobic exercise performed following RYGB surgery elicits further improvement in insulin sensitivity, which may be due to enhanced mitochondrial respiration. We also observed unique weight loss specific (time) effects on mitochondrial respiratory performance.

Trial Registration. clinicaltrials.gov identifier: NCT00692367. Funded by the National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases (R01 DK078192).


O2k-Network Lab: US FL Orlando Goodpaster BH


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, mt-Medicine, Patients  Pathology: Diabetes 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 


Pathway: N, NS  HRR: Oxygraph-2k  Event: A3, Oral  MiP2014: Huntington's 

Affiliation

1-Translat Research Inst Metabol Diabetes, Florida Hosp, Orlando FL; 2-Div Endocrinology Metabolism, Dep Medicine, Univ Pittsburgh, PA; 3-Dep Kinesiology, East Carolina Univ, Greenville, NC; 4-Dep Surgery, Allegheny Health Network, Pittsburgh, PA; USA. - paul.coen@flhosp.org