Description
Coupling-control ratios CCR are flux control ratios FCR at a constant mitochondrial pathway-control state. In mitochondrial preparations, there are three well-defined coupling states of respiration: LEAK respiration, OXPHOS, and Electron-transfer-pathway state (ET state). In these states, the corresponding respirtory rates are symbolized as L, P, and E. In living cells, the OXPHOS state cannot be induced, but in the ROUTINE state the respiration rate is R. A reference rate Z is defined by taking Z as the maximum flux, i.e. flux E in the ET-state, such that the lower and upper limits of the CCR are defined as 0.0 and 1.0. Then there are two mitochondrial CCR, L/E and P/E, and two CCR for living cells, L/E and R/E.
Abbreviation: CCR
Reference: Flux control ratio, Gnaiger 2020 BEC MitoPathways
References
Bioblast link | Reference | Year |
---|---|---|
Gnaiger 2020 BEC MitoPathways | Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | 2020 |
BEC 2020.1 doi10.26124bec2020-0001.v1 | Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 | 2020 |
Keywords
4-compartmental OXPHOS model. (1) ET capacity E of the noncoupled electron transfer system ETS. OXPHOS capacity P is partitioned into (2) the dissipative LEAK component L, and (3) ADP-stimulated P-L net OXPHOS capacity. (4) If P-L is kinetically limited by a low capacity of the phosphorylation system to utilize the protonmotive force pmF, then the apparent E-P excess capacity is available to drive coupled processes other than phosphorylation P» (ADP to ATP) without competing with P».
- Bioblast links: Coupling control - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
1. Mitochondrial and cellular respiratory rates in coupling-control states
Respiratory rate | Defining relations | Icon | |
---|---|---|---|
OXPHOS capacity | P = P´-Rox | mt-preparations | |
ROUTINE respiration | R = R´-Rox | living cells | |
ET capacity | E = E´-Rox | » Level flow | |
» Noncoupled respiration - Uncoupler | |||
LEAK respiration | L = L´-Rox | » Static head | |
» LEAK state with ATP | |||
» LEAK state with oligomycin | |||
» LEAK state without adenylates | |||
Residual oxygen consumption Rox | L = L´-Rox |
2. Flux control ratios related to coupling in mt-preparations and living cells
FCR | Definition | Icon | |
---|---|---|---|
L/P coupling-control ratio | L/P | » Respiratory acceptor control ratio, RCR = P/L | |
L/R coupling-control ratio | L/R | ||
L/E coupling-control ratio | L/E | » Uncoupling-control ratio, UCR = E/L (ambiguous) | |
P/E control ratio | P/E | ||
R/E control ratio | R/E | » Uncoupling-control ratio, UCR = E/L | |
net P/E control ratio | (P-L)/E | ||
net R/E control ratio | (R-L)/E |
3. Net, excess, and reserve capacities of respiration
Respiratory net rate | Definition | Icon |
---|---|---|
P-L net OXPHOS capacity | P-L | |
R-L net ROUTINE capacity | R-L | |
E-L net ET capacity | E-L | |
E-P excess capacity | E-P | |
E-R reserve capacity | E-R |
4. Flux control efficiencies related to coupling-control ratios
Coupling-control efficiency | Definition | Icon | Canonical term | ||
---|---|---|---|---|---|
P-L control efficiency | jP-L | = (P-L)/P | = 1-L/P | P-L OXPHOS-flux control efficiency | |
R-L control efficiency | jR-L | = (R-L)/R | = 1-L/R | R-L ROUTINE-flux control efficiency | |
E-L coupling efficiency | jE-L | = (E-L)/E | = 1-L/E | E-L ET-coupling efficiency » Biochemical coupling efficiency | |
E-P control efficiency | jE-P | = (E-P)/E | = 1-P/E | E-P ET-excess flux control efficiency | |
E-R control efficiency | jE-R | = (E-R)/E | = 1-R/E | E-R ET-reserve flux control efficiency |
5. General
- » Basal respiration
- » Dyscoupled respiration
- » Dyscoupling
- » Electron leak
- » Electron-transfer-pathway state
- » Hyphenation
- » Oxidative phosphorylation
- » OXPHOS analysis
- » Oxygen flow
- » Oxygen flux
- » Permeabilized cells
- » Phosphorylation system
- » Proton leak
- » Proton slip
- » Respiratory state
- » Uncoupling
- Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Quantities for normalization
- » Count in contrast to Number
- » Mitochondrial marker
- » Mitochondrial and marker-enzymes
- » Citrate synthase activity
- Quantities for normalization
- General
- Related keyword lists
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry