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Creatine is a nitrogenous organic acid that occurs naturally in vertebrates and helps primarily muscle cells to supply energy by increasing the formation of adenosine triphosphate (ATP).

Abbreviation: Cr

Reference: Saks 2000 Acta Physiol Scand

Application in HRR

Cr: Creatine (creatine monohydrate, C4H9N3O2*H2O ); M = 149.15 g·mol-1; Sigma 27900, 100 g, store at RT.

Preparation of MiR05Cr or MiR06Cr with 20 mM creatine:
Caution: Prepare fresh everyday!
  1. Weigh 6 mg creatine.
  2. Dissolve in 2 mL MiR05 or MiR06 to obtain MiR05Cr or MiR06Cr.

MitoPedia topics: Substrate and metabolite