Davis 2024 BEC

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Gnaiger 2020 BEC MitoPathways
Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1.
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Davis MS, Barrett MR, Bayly WM, Bolinger A (2024) Effect of selected fluorophores on equine skeletal muscle mitochondrial respiration. Bioenerg Commun 2024.2. https://doi.org/10.26124/bec:2024-0002

Β» Bioenerg Commun 2024.02. Open Access pdf
published online 2024-04-25

Davis Michael S, Barrett Montana R, Bayly Warwick M, Bolinger Amanda (2024) Bioenerg Commun

Abstract: BEC.png https://doi.org/10.26124/bec:2024-0002

Equine skeletal muscle provides a rich source of mitochondria, suitable for both basic and applied studies of cellular energetics. Inclusion of fluorophores and the associated endpoints provides valuable information to complement measurements of mitochondrial oxygen flux, but investigators must be aware of the possible artifacts created by those fluorophores. Our studies demonstrate that Magnesium Green has no significant effect on respiration of isolated mitochondria, whereas tetramethylrhodamine inhibits phosphorylating respiration and increases LEAK respiration. Amplex UltraRed increases phosphorylating and non-phosphorylating respiration through Complex I. The effect of Amplex UltraRed is a novel finding, and further study is necessary to determine the mechanism underlying this artifact. β€’ Keywords: horse, Amplex UltraRed, Magnesium Green, Tetramethylrhodamine, LEAK respiration β€’ Bioblast editor: Tindle-Solomon L β€’ O2k-Network Lab: US OK Stillwater Davis MS

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MitoFit Preprint

Β» Davis 2023 MitoFit

Labels: MiParea: Respiration 

Organism: Horse  Tissue;cell: Skeletal muscle  Preparation: Isolated mitochondria 

Coupling state: LEAK, OXPHOS  Pathway: N, S  HRR: Oxygraph-2k 

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