Dias Candida

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BEC 2020.1 Mitochondrial physiology
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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

Dias Candida

MitoPedia topics: EAGLE 

COST: Member COST WG1: WG1


Name Dias Cândida, PhD student
Candida D
Redox Biology and Brain Sensing,

Center for Neuroscience and Cell Biology,

University of Coimbra, PT

Address Rua Larga - Faculdade de Medicina, Pólo 1, Piso 1, 3004-504
City Coimbra
Country Portugal
Email [email protected]
O2k-Network Lab PT Coimbra Laranjinha J



Dias 2023 Biofactors2023Dias C, Fernandes E, Barbosa RM, Laranjinha J, Ledo A (2023) Astrocytic aerobic glycolysis provides lactate to support neuronal oxidative metabolism in the hippocampus. https://doi.org/10.1002/biof.1951
Dias 2022 Free Radic Biol Med2022Dias C, Lourenço CF, Laranjinha J, Ledo A (2022) Modulation of oxidative neurometabolism in ischemia/reperfusion by nitrite. https://doi.org/10.1016/j.freeradbiomed.2022.11.021
BEC 2020.1 doi10.26124bec2020-0001.v12020Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
Dias 2018 Anal Biochem2018Dias C, Lourenco CF, Barbosa RM, Laranjinha J, Ledo A (2018) Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices. Anal Biochem 551:43-50.
Dias 2016 Neurobiol Aging2016Dias C, Lourenço CF, Ferreiro E, Barbosa RM, Laranjinha J, Ledo A (2016) Age-dependent changes in the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic model of Alzheimer's disease: implications for neurometabolic regulation. Neurobiol Aging 46:84-95.
Lourenco 2015 Front Aging Neurosci2015Lourenço CF, Ledo A, Dias C, Barbosa RM, Laranjinha J (2015) Neurovascular and neurometabolic derailment in aging and Alzheimer's disease. Front Aging Neurosci 7:103.


Dias 2019 MiPschool Coimbra2019
Candida Dias
Protective effect of nitrite in brain ischemia/reperfusion via modulation of mitochondrial respiration.

MitoEAGLE Short-Term Scientific Mission

STSM Grant Period 4
Work Plan summary
Nitrite, once considered an inert metabolic endpoint of nitric oxide (•NO), has more recently emerged as a metabolic precursor of •NO in vivo. This alternative source of •NO may play a critical role in the brain under emergency conditions such as ischemia, when enzymatic •NO production is hindered due to lack of oxygen. Evidence shows that nitrite is protective in situations of ischemia/reperfusion and appears to be beneficial in aging and neurodegeneration. Most relevantly, nitrite concentration in vivo can be modulated by diet through the ingestion of nitrate rich foods, which are generally associated with increased longevity and lower incidence of cardiovascular disease. One putative target for nitrite´s protective bioactivity in ischemia is through modulation of mitochondrial respiration. In our previous work, we applied an in vitro ischemia/reperfusion protocol to permeabilised rat hippocampal tissue and determined the differences of NADH-linked respiration (supported by glutamate, pyruvate and malate) in the presence and absence of nitrite. Our preliminary results indicate that while under control conditions (no nitrite), a significant increase in the respiration rate is observed upon re-oxygenation (“oxidative burst”), in the presence of nitrite (10 µM) this burst is abolished. However, this effect is dose-dependent, as a higher concentration (100 µM) could not prevent the oxidative burst. This inhibition may prevent the increased production of reactive oxygen species associated with this oxidative burst and may be one of the mechanisms through which nitrite is protective during brain ischemia. The aim of this STSM will be to determine the effects of nitrite on brain mitochondrial respiration and hydrogen peroxide production upon ischemia/reperfusion, using an identical protocol to what we previously did, to better correlate between measurements. The results could help to understand the mechanism through which nitrite appears to be protective in brain ischemia/reperfusion. Furthermore, it will include the evaluation of a jointly optimized tissue holder to extend SOPs from tissue homogenates, isolated mitochondria and permeabilized tissue to standardized and harmonized protocols and applications with tissue slices. Before starting this experimental plan, this mission will involve the participation in the “Oroboros O2k-Workshop” where an update on latest developments of high-resolution respirometry, including fluorometry and TiP2k applications for evaluation of mitochondrial function, will be provided.

Participated at

Visiting scientist in the Oroboros O2k-Laboratory


Dias Candida: Visiting scientist at the Oroboros O2k-Laboratory

  • September 23 to December 21 2019
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