Dias C

From Bioblast
Jump to: navigation, search


e-COST MitoEAGLE
e-COST MitoEAGLE countries
MitoGlobal
MitoEAGLE
News and Events         About COST Action MitoEAGLE         Working Groups         MiP/MitoEAGLE Conference, WG and MC Meeting 2019 Belgrade RS         MitoEAGLE Preprint States and rates         Short-Term Scientific Missions         Inclusiveness Target Countries         Management Committee         Members    


MiPsociety
COST Action CA15203 MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


 

Dias C


MitoPedia topics: EAGLE 

COST: Member COST WG1: WG1

COST WG3: WG3


Name Dias Cândida, PhD student
Institution
Candida D
Redox Biology and Brain Sensing,

Center for Neuroscience and Cell Biology,

University of Coimbra, PT

Address Rua Larga - Faculdade de Medicina, Pólo 1, Piso 1, 3004-504
City Coimbra
State/Province
Country Portugal
Email candidamndias@gmail.com
Weblink
O2k-Network Lab PT Coimbra Laranjinha J


Labels:



Publications

 PublishedReference
Gnaiger 2019 MitoFit Preprint Arch2019Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v6.
Dias 2018 Anal Biochem2018Dias C, Lourenco CF, Barbosa RM, Laranjinha J, Ledo A (2018) Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices. Anal Biochem 551:43-50.
Dias 2016 Neurobiol Aging2016Dias C, Lourenço CF, Ferreiro E, Barbosa RM, Laranjinha J, Ledo A (2016) Age-dependent changes in the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic model of Alzheimer's disease: implications for neurometabolic regulation. Neurobiol Aging 46:84-95.
Lourenco 2015 Front Aging Neurosci2015Lourenço CF, Ledo A, Dias C, Barbosa RM, Laranjinha J (2015) Neurovascular and neurometabolic derailment in aging and Alzheimer's disease. Front Aging Neurosci 7:103.

Abstracts

 PublishedReference
Dias 2019 MiPschool Coimbra2019
Candida Dias
Protective effect of nitrite in brain ischemia/reperfusion via modulation of mitochondrial respiration.


MitoEAGLE Short-Term Scientific Mission

Work Plan summary
Nitrite, once considered an inert metabolic endpoint of nitric oxide (•NO), has more recently emerged as a metabolic precursor of •NO in vivo. This alternative source of •NO may play a critical role in the brain under emergency conditions such as ischemia, when enzymatic •NO production is hindered due to lack of oxygen. Evidence shows that nitrite is protective in situations of ischemia/reperfusion and appears to be beneficial in aging and neurodegeneration. Most relevantly, nitrite concentration in vivo can be modulated by diet through the ingestion of nitrate rich foods, which are generally associated with increased longevity and lower incidence of cardiovascular disease. One putative target for nitrite´s protective bioactivity in ischemia is through modulation of mitochondrial respiration. In our previous work, we applied an in vitro ischemia/reperfusion protocol to permeabilised rat hippocampal tissue and determined the differences of NADH-linked respiration (supported by glutamate, pyruvate and malate) in the presence and absence of nitrite. Our preliminary results indicate that while under control conditions (no nitrite), a significant increase in the respiration rate is observed upon re-oxygenation (“oxidative burst”), in the presence of nitrite (10 µM) this burst is abolished. However, this effect is dose-dependent, as a higher concentration (100 µM) could not prevent the oxidative burst. This inhibition may prevent the increased production of reactive oxygen species associated with this oxidative burst and may be one of the mechanisms through which nitrite is protective during brain ischemia. The aim of this STSM will be to determine the effects of nitrite on brain mitochondrial respiration and hydrogen peroxide production upon ischemia/reperfusion, using an identical protocol to what we previously did, to better correlate between measurements. The results could help to understand the mechanism through which nitrite appears to be protective in brain ischemia/reperfusion. Furthermore, it will include the evaluation of a jointly optimized tissue holder to extend SOPs from tissue homogenates, isolated mitochondria and permeabilized tissue to standardized and hormonized protocols and applications with tissue slices. Before starting this experimental plan, this mission will involve the participation in the “Oroboros O2k-Workshop” where an update on latest developments of high resolution respirometry, including fluorometry and TiP2k applications for evaluation of mitochondrial function, will be provided.


Participated at


Visiting scientist in the Oroboros MitoFit Laboratory

O2k-Network

Dias Candida: Visiting scientist at the Oroboros MitoFit Laboratory

  • September 23 to December 21 2019