Drahota 2005 Cell Biochem Biophys

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Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Development of cytochrome-c oxidase in rat heart. Cell Biochem Biophys 43:87-94.

» PMID: 16043886

Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Cell Biochem Biophys

Abstract: Cytochrome c oxidase (COX) activity of the rat heart was two- to sevenfold activated when the membrane integrity was disrupted by hypotonic swelling, freezing-thawing, or a detergent, indicating that a large portion of COX capacity in intact mitochondria is not active. The effect of detergent was tested in heart mitochondria isolated from 1-, 5-, 15-, 29-, and 60-d-old rats; activation by detergent was up to 20-fold in 1-d-old animals, whereas in mitochondria from 60-d-old rats it was only 7-fold. Our data indicate that the rat heart exhibits significant developmental changes dependent on downregulation of COX activity in the neonatal period.

Keywords: Rat heart, Postnatal development, Cytochrome-c oxidase, Mitochondria, Detergent

O2k-Network Lab: CZ Prague Houstek J


Labels: MiParea: Respiration 


Organism: Rat  Tissue;cell: Heart  Preparation: Isolated mitochondria, Oxidase;biochemical oxidation  Enzyme: Complex IV;cytochrome c oxidase 

Coupling state: OXPHOS 

HRR: Oxygraph-2k