Endlicher 2009 Physiol Res

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Endlicher R, Kriváková P, Rauchová H, Nůsková H, Cervinková Z, Drahota Z (2009) Peroxidative damage of mitochondrial respiration is substrate-dependent. Physiol Res 58:685-92.

» PMID: 19093725 Open Access

Endlicher R, Krivakova P, Rauchova H, Nuskova H, Cervinkova Z, Drahota Z (2009) Physiol Res

Abstract: The concentration-dependence of tert-butyl hydroperoxide (BHP) inhibitory effect on oxygen consumption in isolated rat liver mitochondria was measured in the presence of various respiratory substrates. Strong inhibitory effect at low concentrations of BHP (15-30 microM) was found for oxoglutarate and palmitoyl carnitine oxidation. Pyruvate and glutamate oxidation was inhibited at higher concentrations of BHP (100-200 microM). Succinate oxidation was not affected even at 3.3 mM BHP. Determination of mitochondrial membrane potential has shown that in the presence of NADH-dependent substrates the membrane potential was dissipated by BHP but was completely restored after addition of succinate. Our data thus indicate that beside peroxidative damage of complex I also various mitochondrial NADH-dependent dehydrogenases are inhibited, but to a different extent and with different kinetics. Our data also show that succinate could be an important nutritional substrate protecting hepatocytes during peroxidative damage.

Keywords: Liver mitochondria, Mitochondrial enzymes, Peroxidative damage, Tert-butyl hydroperoxide

O2k-Network Lab: CZ Hradec Kralove Cervinkova Z, CZ Prague Houstek J


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Organism: Rat  Tissue;cell: Liver  Preparation: Isolated mitochondria 



HRR: Oxygraph-2k