Endlicher 2016 Physiol Res

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Endlicher R, Drahota Z, Cervinkova Z (2016) In vitro and in vivo activation of mitochondrial membrane permeability transition pore using triiodothyronine. Physiol Res 65:321-31.

» PMID: 26447515 Open Access

Endlicher R, Drahota Z, Cervinkova Z (2016) Physiol Res

Abstract: Using a novel method for evaluating mitochondrial swelling (Drahota et al. 2012a) we studied the effect of calcium (Ca(2+)), phosphate (P(i)), and triiodothyronine (T(3)) on the opening of mitochondrial membrane permeability transition pore and how they interact in the activation of swelling process. We found that 0.1 mM P(i), 50 microM Ca(2+) and 25 microM T(3) when added separately increase the swelling rate to about 10 % of maximal values when all three factors are applied simultaneously. Our findings document that under experimental conditions in which Ca(2+) and P(i) are used as activating factors, the addition of T(3) doubled the rate of swelling. T(3) has also an activating effect on mitochondrial membrane potential. The T(3) activating effect was also found after in vivo application of T(3). Our data thus demonstrate that T(3) has an important role in opening the mitochondrial membrane permeability pore and activates the function of the two key physiological swelling inducers, calcium and phosphate ions.

Keywords: Rat liver mitochondria, Membrane permeability transition pore, Thyroid hormones

O2k-Network Lab: CZ Hradec Kralove Cervinkova Z


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Tissue;cell: Liver  Preparation: Isolated mitochondria 

Regulation: Calcium, mt-Membrane potential