Flores-Herrera 2019 Protoplasma

Flores-Herrera C, Preciado-Linares G, Gonzalez-Vizueth I, Corona de la Peña N, Gutiérrez-Aguilar M (2019) In situ assessment of mitochondrial calcium transport in tobacco pollen tubes. https://doi.org/10.1007/s00709-018-1316-z

» Protoplasma 256:503-509. PMID: 30288611

Flores-Herrera Cesar, Preciado-Linares Gisela, Gonzalez-Vizueth Israel, Corona de la Pena Norma, Gutierrez-Aguilar Manuel (2019) Protoplasma

Abstract: Pollen tubes require functional mitochondria in order to achieve fast and sustained growth. In addition, cell wall expansion requires a calcium gradient in the tube apex formed by a dedicated array of calcium pumps and channels. Most studies have traditionally focused on the molecular aspects of calcium interactions and transport across the pollen tube plasmalemma. However, calcium transients across mitochondrial membranes from pollen tubes are beginning to be studied. Here, we report the presence of a ruthenium red-sensitive mitochondrial calcium uniporter-like activity in tobacco pollen tubes with functional oxidative phosphorylation. The present study provides a framework to measure in situ specifics of mitochondrial transport and respiration in pollen tubes from different plants. The relevance of a mitochondrial calcium uniporter for pollen tube growth is discussed. • Keywords: Digitonin, Mitochondrial calcium uniporter, Nicotiana, Pollen tubes, Transport • Bioblast editor: Plangger M

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