Garcia-Roves 2017 MiPschool Obergurgl

From Bioblast
Mitochondrial fitness in skeletal muscle.

Link: MitoEAGLE

Garcia-Roves PM (2017)

Event: MiPschool Obergurgl 2017


Skeletal muscles respond to environmental changes through integration of signals sent by other tissues, nutritional cues, and/or those initiated by local stimuli. Thus, we can observe diverse skeletal muscle phenotypes due to chronic environmental adaptations (inactivity versus exercise), disease or traumatic injury. Stimuli such as exercise training induces regular myofibrillar contraction, which perturbs: redox state, oxygen partial pressure, energy supply, substrate type and abundance, production of reactive oxygen species, calcium flux, and invokes mechanical stress [1]. Homeostatic disruption activates signalling cascades, which facilitate metabolic and morphological adaptations through the direct activation of enzymes, transcriptional regulation or post-translational modifications [2,3,4].

One of the major metabolic adaptations due to skeletal muscle use and disuse are related to mitochondrial fitness. Mitochondria are a dynamic organellar network that reacts to homeostatic disturbances to adjust energy demands and cataplerotic and anaplerotic reactions to cellular needs. During this presentation, several examples related to mitochondrial responses to exercise, transgenesis and chronic metabolic diseases will be presented. Skeletal muscle tissue processing for high-resolution respirometry, SUIT protocols and integration of data results will be emphasized during this talk.

β€’ Bioblast editor: Kandolf G β€’ O2k-Network Lab: ES Barcelona Garcia-Roves PM, ES Barcelona IDIBAPS Hospital Clinic

Labels: MiParea: Respiration, Comparative MiP;environmental MiP, Exercise physiology;nutrition;life style, mt-Medicine 

Tissue;cell: Skeletal muscle 

HRR: Oxygraph-2k  Event: E1, Review 


Univ Barcelona, Fac Medicine Health Sciences, Dept Physiological Sciences; Bellvitge Biomedical Research Inst (IDIBELL) Barcelona, Spain.- [email protected]


  1. Egan B, Zierath JR (2013) Exercise metabolism and the molecular regulation of skeletal muscle adaptation. Cell Metab 17:162-84.
  2. Garcia-Roves PM, Osler ME, HolmstrΓΆm MH, Zierath JR (2008) Gain-of-function R225Q mutation in AMP-activated protein kinase gamma3 subunit increases mitochondrial biogenesis in glycolytic skeletal muscle. J Biol Chem 283:35724-34.
  3. Garcia-Roves P, Huss JM, Han DH, Hancock CR, Iglesias-Gutierrez E, Chen M, Holloszy JO (2007) Raising plasma fatty acid concentration induces increased biogenesis of mitochondria in skeletal muscle. Proc Natl Acad Sci U S A 104:10709-13.
  4. Deshmukh AS (2016) Proteomics of skeletal muscle: focus on insulin resistance and exercise biology. Proteomes 4pii:E6.
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