Gnaiger 1997 Transplant Proc
|Gnaiger E, Rieger G, Kuznetsov A, Fuchs A, Stadlmann S, Lassnig B, Hengster P, Eberl T, Margreiter R (1997) Mitochondrial ischemia-reoxygenation injury and plasma membrane integrity in human endothelial cells. Transplant Proc 29:3524-6.|
Abstract: 0RGAN PRESERVATION under hypothermic ischemia is enhanced by storage solutions that protect the vascular endothelium from ischemia-reperfusion injury. Ischemia-reperfusion injury leads to primary graft failure and chronic rejection, and is commonly assessed by measuring endothelial activation and damage of the endothelial plasma membrane. However, corresponding primary intracellular events are little understood compared with the secondary cytokine/adhesion molecule cascade and inflammatory responses.1, 2 Because protection of intracellular and cell membrane function is fundamental for further improvement of organ preservation, we developed highresolution respirometry as a sensitive diagnostic test for mitochondrial and plasma membrane competence.3 Whereas the plasma membrane remained impermeable after clinically relevant cold storage times of 8 hours and 20 to 60 minutes of reoxygenation, mitochondrial function was impaired at several steps of the respiratory chain.
• O2k-Network Lab: AT Innsbruck Gnaiger E
Stress:Ischemia-reperfusion Organism: Human Tissue;cell: Endothelial;epithelial;mesothelial cell Preparation: Intact cells
Coupling state: OXPHOS