Gnaiger 2016 Abstract UMDF2016
|Schöpf B, Schäfer G, Weber A, Talasz H, Eder IE, Klocker H, Gnaiger E (2016) Oxidative phosphorylation and mitochondrial function differ between human prostate tissue and cultured cells.|
Cell lines are frequently used as models to study mitochondrial (dys)function but little is known about their mitochondrial respiration and metabolic function in comparison to the primary tissue of origin. Oxidative phosphorylation (OXPHOS) was studied in immortalized prostate epithelial and fibroblast cell lines using high-resolution respirometry. RWPE1 and NAF, representing the major cell types in prostate tissue, were compared to the tissue of origin, with prostate tissue samples of only 2 mg wet weight. Substrate-uncoupler-inhibitor titration (SUIT) protocols were applied to investigate the respiratory activity of different segments of the OXPHOS system in mechanically permeabilized tissue biopsies. The cell lines represented the bioenergetic properties of fresh tissue to a limited extent only. Particularly, tissue showed a higher oxidative capacity with succinate and glutamate, whereas pyruvate was a substrate supporting significantly higher respiratory activities in cell lines. Several fold higher zinc levels measured in tissue compared to cells confirmed the role of aconitase for prostate specific metabolism in agreement with observed respiratory properties. In conclusion, combining the flexibility of cell culture models and tissue samples for respirometric analysis are powerful tools for investigation of mitochondrial function and tissue specific metabolism.
• Keywords: Prostate metabolism, Mitochondria, High-resolution respirometry, Oxidative phosphorylation, electron transfer-pathway
Labels: MiParea: Respiration, Comparative MiP;environmental MiP, mt-Medicine
Organism: Human Tissue;cell: Endothelial;epithelial;mesothelial cell, Genital, Other cell lines, Fibroblast Preparation: Permeabilized cells, Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET Pathway: F, N, NS HRR: Oxygraph-2k
1-Division Genetic Epidemiology, Dept Med Genetics, Molecular Clinical Pharmacology, Medical Univ Innsbruck; 2-Division Experimental Urology, Dept Urology, Medical Univ Innsbruck; 3-Dept Pathology, Medical Univ Innsbruck; 4-Biocenter, Section Clinical Biochem, Medical Univ Innsbruck; 5-Dept General Transplant Surgery, D. Swarovski Research Lab, Medical Univ Innsbruck, Austria. - [email protected]
Funded by the Austrian FFG (Oncotyrol), Tyrolean Future Foundation, and K-Regio MitoFit. Contribution to COST Action MITOEAGLE.