Hand 1990 J Comp Physiol B

From Bioblast
Publications in the MiPMap
Hand SC (1990) Heat dissipation during long-term anoxia in Artemia franciscana embryos: Identification and fate of metabolic fuels. J Comp Physiol B 160:357-63.

Β» PMID: 2292613

Hand SC (1990) J Comp Physiol B

Abstract: Microcalorimetric measurements of brine shrimp embryos during 6 days of anoxia indicated that heat dissipation was rapidly suppressed to 2.7% of control (aerobic) values over the first 9 h. Energy flow continued to decline slowly to 31 Β΅WΒ·g dry mass-1 (0.4% of control) during the subsequent 5.5 days. Within 2 h after returning anoxic embryos to aerobic conditions, heat dissipation rose to 77% of control rates. The calorimetric/respirometric (CR) ratio across this 2-h recovery period increased steadily from-226 to-346 kJΒ·mol O2 -1 . Prior to the anoxic exposures, hydrated embryos were incubated aerobically for 10 h to insure full initiation of carbohydrate metabolism (CR ratio=-484 kJΒ·mol O2 -1 ). During the 6-day asymptotic approach to a nearly ametabolic state, trehalose and glycogen levels declined 18% and 13%, respectively. The majority of this utilization occurred within the first three days. Thermochemical calculations showed that carbohydrate catabolism accounted for 84% of the total heat dissipation measured over the 6-day anoxic bout; only 3% of the heat could be explained by the catabolism of diguanosine tetraphosphate (Gp4G). Analyses of embryo extracts by high performance liquid chromatography indicated that multiple acid end products were accumulated. Lactate and propionate reached 4.5 mM and 1.0 mM, respectively, but these compounds did not account quantitatively for the amount of carbohydrate utilized. However, the largest chromatographic peak that accumulated under anoxia has not been successfully identified. Fumarate and pyruvate levels decreased as anoxia proceeded. Thus, a perceptible energy flow in Artemia franciscana embryos still remained after 6 days of anoxia. While an ametabolic state may be reached with time, the length of this prolonged transition into anaerobic dormancy has not been appreciated before. β€’ Keywords: Twin-Flow, Anaerobic dormancy, Anoxia, Artemia, Calorimetry

β€’ O2k-Network Lab: US LA Baton Rouge Hand SC

Labels: MiParea: Respiration, Comparative MiP;environmental MiP 

Stress:Ischemia-reperfusion  Organism: Artemia, Crustaceans 

Preparation: Intact organism 

Regulation: Substrate  Coupling state: ROUTINE 

CaloRespirometry, Twin-Flow 

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