Hatefi 1962 J Biol Chem-XLI

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Hatefi Y, Haavik AG, Griffiths DE (1962) Studies on the electron transfer system. XLI. Reduced coenzyme Q (QH2)-cytochrome c reductase. J Biol Chem 237:1681-5.

» PMID: 13905328 Open Access; PDF

Hatefi Y, Haavik AG, Griffiths DE (1962) J Biol Chem

Abstract: The preparation from beef heart mitochondria and the properties, of a highly active and stable enzyme system, capable of catalyzing the reduction of cytochrome c by reduced coenzyme Q2 are described. The enzyme complex contains cytochrome b and cytochrome c1 in high concentration, and is free of cytochrome c oxidase, cytochrome c, flavoproteins, and the citric acid cycle dehydrogenases. The activity of the enzyme corresponds to a QO2, of about 320,000 at 38°. This activity is strongly inhibited by antimycin A, 2-nonyl-4-hydroxy-quinoline-N-oxide, and 2-alkyl-3-hydroxynaphthoquinone. Amytal, thenoyltrifluoroacetone, and a number of specific metal-chelating compounds are ineffective as inhibitors. Keywords: Electron transfer-pathway, Q-junction, Coenzyme Q, Reduced coenzyme Q (QH2), Cytochrome c reductase, Beef heart

Cited by

Gnaiger 2020 BEC MitoPathways

Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002


Organism: Bovines  Tissue;cell: Heart  Preparation: Isolated mitochondria  Enzyme: Complex IV;cytochrome c oxidase  Regulation: Substrate  Coupling state: ET 

Made history, BEC 2020.2