Huete-Ortega 2018 AlgaEurope2018
|Huete-Ortega M, Komlodi T, Pranger F, Gnaiger E (2018) High-resolution respirometry: new perspectives for the study of microalgae bioenergetics and applications in biotechnology. AlgaEurope2018.|
Event: ALGAEUROPE 2018 Amsterdam NL
Bioenergetics is the study of energy transformations in cells, mitochondria, and chloroplasts as a basis to perform biochemical work. In algal chloroplasts solar energy is acquired and assimilated through photosynthesis to synthesize the organic matter required for growth, which is associated with production of oxygen, while mitochondrial respiration consumes oxygen to transform organic matter into the chemical energy that fuels cellular activity. Energetic coupling between chloroplasts and mitochondria has been described in algae. For example, mitochondrial physiology is involved in mitigating light stress in the photosynthetic pathway or chloroplast-generated reducing equivalents replace photophosphorylation as a source of ATP in the mitochondria. Therefore, a good functionality and cross-talk between both organelles is necessary to maintain metabolic integrity. High-resolution respirometry (HRR) is widely used to assess mitochondrial respiration and other bioenergetic parameters of isolated mitochondria, cultured cells, tissue preparations and human biopsies. In the biomedical field of mitochondrial physiology and its clinical applications, studies with HRR relate to degenerative diseases and life style-linked preventive medicine. I propose to extend the experimental options of the Oroboros O2k developed by Oroboros Instruments for HRR to the study of algae bioenergetics for biotechnological purposes. Outstanding applications include: i) research on mitochondrial respiration, photosynthesis and other bioenergetic parameters in algae, including the metabolic interactions between mitochondria and chloroplasts and their effects on production of biomass and organic molecules; ii) analysis of functional alterations of algae mutants generated for biotechnology; iii) optimization of cryopreservation in algae strains used in biotechnology by diagnostic evaluation of bioenergetic functionality.
Labels: MiParea: Respiration
Huete-Ortega M(1,2), Komlodi T(1), Pranger F(1), Gnaiger E(1)
- Oroboros Instruments GmbH, Innsbruck, Austria. - [email protected]
- Dept Plant Sciences, Univ Cambridge, Cambridge, UK