Huete-Ortega 2020 AlgaEurope2020

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Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E (2020) Application of high-resolution respirometry for studying chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii grown for biotechnology exploitations. AlgaEurope 2020 Rome IT.

Link: European Algae Biomass Association

Huete-Ortega Maria, Di Marcello Marco, Iglesias-Gonzalez Javier, Gnaiger Erich (2020)

Event: AlgaEurope 2020 Rome IT

Chloroplast and mitochondrial bioenergetic control of algal growth and metabolite production is necessary to maintain metabolic integrity. In contrast to mammalian cells, algal mitochondria express alternative oxidases (AOX), which divert electron transfer from coenzyme Q (Q) away from cytochrome c oxidase (Complex IV, CIV) [1]. In algae, therefore, we distinguish between respiration through the Q-AOX and Q-CIV branches of the electron transfer system. A better understanding of the contribution of both branches to mitochondrial dark respiration is required to optimize algal biomass and metabolite production for biotechnological purposes. High-resolution respirometry (HRR; Oroboros O2k) is widely used to assess mitochondrial respiration and other bioenergetic parameters in the biomedical field of mitochondrial and cell research [2]. In our interdisciplinary study, we introduced a new method of studying oxygen kinetics [3] for the partitioning of dark respiration between the Q-AOX and Q-CIV branches in Chlamydomonas reinhardtii grown for biomass and lipid production [4]. This method is based on the distinct oxygen affinities of these enzymes. In addition, the multimodal approach of the Oroboros O2k was extended using the novel PhotoBiology-Module of the NextGen-O2k for determining the maximum light-saturated and inorganic carbon-saturated photosynthesis rates [5,6]. This methodology allows for increasing our current knowledge of the bioenergetics of chloroplasts and mitochondria, which is of particular importance for biotechnological exploitations.

Keywords: Chlamydomonas reinhardtii, High-resolution respirometry, Lipids, Biomass, Oxygen kinetics Bioblast editor: Plangger M O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck Gnaiger E

Labels: MiParea: Respiration, Instruments;methods 

Organism: Plants 

HRR: Oxygraph-2k 

Affiliations and support

Oroboros Instruments GmbH, Innsbruck, Austria
This communication is part of the NextGen-O2k project that has received funding from the European Union’s Horizon 2020 research and innovation program under the grant agreement No 859770.


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