Karabatsiakis 2014 Abstract IOC96
|Karabatsiakis A (2014) Consequences of depression on respiratory activity in peripheral blood mononuclear cells. Mitochondr Physiol Network 19.11.|
Stress and trauma are contributing factors to the etiology and pathophysiology of depression. Although the precise underlying biological mechanisms are not completely known, the role of mitochondrial physiology reaches increasing focus of attention in this context. Clinical symptoms of depression include a lack of energy and motivation, sleep disturbances, cognitive deficits and reduced immunity, all linking to changes in energy metabolism. In this study we investigated the respiratory activity of peripheral blood mononuclear cells (PBMCs), an established model to investigate the psychoimmunological consequences of depression.
In total 44 participants (n=22 acute depressed patients, n=22 non-depressed healthy controls) participated in the study. Peripheral blood was collected by venous punction and PBMCs were isolated using Ficoll-based dense gradient centrifugation following storage at -80° C until analysis. After thawing cells were transferred into an Oroboros high-resolution oxygraph for the characterization of respiratory activity.
PBMCs of acute depressed subjects showed significantly lower respiratory activity: routine and uncoupled respiration as well as spare respiratory capacity, coupling efficiency and ATP turnover-related respiration were significantly decreased. Further, impaired respiration of PBMCs showed a significantly negative correlation with clinical symptom severity of depression.
Here we not only show that mitochondrial activity in immune cells is impaired in acute depressed patients but also that symptom severity is significantly correlated with mitochondrial respiration. Our results strengthen the perspective of a contributing role of mitochondria to the pathophysiology of depression. Impaired immunity, a health consequence in depression, might be linked to decreased energy metabolism thus providing a new therapeutical target in the treatment of the disorder an immune-related comorbidities.
• Keywords: PBMC
• O2k-Network Lab: DE Ulm Karabatsiakis A
Labels: MiParea: Respiration, Patients
Organism: Human Tissue;cell: Blood cells, Lymphocyte Preparation: Intact cells
Coupling state: LEAK, ROUTINE, ET