|Karavyraki M, Gnaiger E, Porter RK (2022) Bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. https://doi.org/10.26124/bec:2022-0011|
In an endeavor to understand the metabolic phenotype behind oral squamous cell carcinomas, we characterized the bioenergetic profile of a human tongue derived cancer cell line (SCC-4 cells) and compared this profile to a pre-cancerous dysplastic oral keratinocyte (DOK) cell line also derived from human tongue. The human SCC-4 cancer cells had greater mitochondrial abundance but lower mitochondrial oxygen consumption rates than DOK cells. The lower oxygen consumption rate in SCC-4 cells can be partially explained by lower NADH-related enzymatic activity and lower mitochondrial complex 1 activity when compared to pre-cancerous DOK cells. In addition, SCC-4 cells have greater extracellular acidification rate (an index of glycolytic flux) when compared to DOK cells. In addition, treatment with recombinant human IL-6 (rhIL-6), known to drive anoikis resistance in SCC-4 cells but not DOK cells, impairs oxygen consumption in SCC-4 but not DOK cells, without affecting mitochondrial abundance. We conclude that SCC-4 cells have a less oxidative phenotype compared to DOK cells and that IL-6 attenuates mitochondrial function in SCC-4 cells while increasing glycolytic flux. • Keywords: oral squamous cancer cells; mitochondria; interleukin 6; dysplastic oral keratinocytes; oxygen consumption • Bioblast editor: Tindle-Solomon L • O2k-Network Lab: AT Innsbruck Oroboros, IE Dublin Porter RK
- Original files are available Open Access at Zenodo repository: https://zenodo.org/record/7325015#.Y3QcOcfP1PY
- Marie Curie Grant TRACT 721906 H2020-MCSA-ITN 2016; COST Action CA15203 MitoEAGLE (2016-2021). We thank Rafael Moreno-Sanchez for a constructive review of our manuscript.
Labels: MiParea: Respiration Pathology: Cancer
Preparation: Permeabilized cells Enzyme: Complex I, Marker enzyme, TCA cycle and matrix dehydrogenases Regulation: Aerobic glycolysis Coupling state: LEAK, ROUTINE, ET Pathway: S, ROX HRR: Oxygraph-2k
Crabtree effect, BEC