Kraft 2004 Thesis Universität Halle-Wittenberg - Germany
|Kraft A (2004) Langzeitstabilität der Mitochondrienfunktion permeabilisierter Muskelfasern in der Diagnostik mitochondrialer Enzephalomyopathien. Thesis Universität Halle-Wittenberg - Germany 74pp.|
Abstract: We optimized the skinned fiber technique and high resolution respirometry developed for the examination of mitochondrial function. Storage of skeletal muscle fibers in ice-cooled storage buffer for 36 hours did not caused a significant change in mitochondrial function as determined by respiration of complex I and II dependent substrates. However, stable mitochondrial function was only guaranteed if cooling was without interruption. The newly established multiple substrate-inhibitor titration provides the possibility to measure complex I dependent functional deficits. This parameter was shown to be independent of the mitochondrial content of skeletal muscle fibers. Furthermore, we showed that shipment of samples in ice-cooled storage buffer by mail did not significantly influence mitochondrial function measured by respirometry.
• Keywords: skinned fiber technique, respirometry, mitochondria, mitochondrial function, substrate-inhibitor-titration, mitochondrial encephalomyopathy, shipment of samples
Labels: MiParea: Respiration, Instruments;methods, mt-Medicine
Organism: Human Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS Pathway: N, S, NS, ROX HRR: Oxygraph-2k