Kruse 2021 Life (Basel)

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Publications in the MiPMap
Kruse CJ, Stern D, Mouithys-Mickalad A, Niesten A, Art T, Lemieux H, Votion DM (2021) In vitro assays for the assessment of impaired mitochondrial bioenergetics in equine atypical myopathy. Life (Basel) 11:719.

» PMID: 34357091 Open Access

Kruse Caroline-J, Stern David, Mouithys-Mickalad Ange, Niesten Ariane, Art Tatiana, Lemieux Helene, Votion Dominique-M (2021) Life (Basel)

Abstract: Equine atypical myopathy is a seasonal intoxication of grazing equids. In Europe, this poisoning is associated with the ingestion of toxins contained in the seeds and seedlings of the sycamore maple (Acer pseudoplatanus). The toxins involved in atypical myopathy are known to inhibit ß-oxidation of fatty acids and induce a general decrease in mitochondrial respiration, as determined by high-resolution respirometry applied to muscle samples taken from cases of atypical myopathy. The severe impairment of mitochondrial bioenergetics induced by the toxins may explain the high rate of mortality observed: about 74% of horses with atypical myopathy die, most within the first two days of signs of poisoning. The mechanism of toxicity is not completely elucidated yet. To improve our understanding of the pathological process and to assess therapeutic candidates, we designed in vitro assays using equine skeletal myoblasts cultured from muscle biopsies and subjected to toxins involved in atypical myopathy. We established that equine primary myoblasts do respond to one of the toxins incriminated in the disease. Keywords: Atypical myopathy, Cell culture, Equine primary myoblasts, High-resolution respirometry, Toxicity assays Bioblast editor: Reiswig R O2k-Network Lab: CA Edmonton Lemieux H, BE Liege Votion DM


Labels: MiParea: Respiration, Pharmacology;toxicology  Pathology: Myopathy 

Organism: Horse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized cells, Intact cells 


Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, S, NS, ROX  HRR: Oxygraph-2k 

2021-08