Labajova 2006 Gen Physiol Biophys

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Lábajová A, Kofránek J, Kriváková P, Cervinková Z, Drahota Z (2006) Tetraphenylphosphonium-Selective Electrode as a Tool for Evaluating Mitochondrial Permeability Transition Pore Function in Isolated Rat Hepatocytes. Gen Physiol Biophys 25:325-31.

» PMID: 17197730 Open Access

Labajova A, Kofranek J, Krivakova P, Cervinkova Z, Drahota Z (2006) Gen Physiol Biophys

Abstract: The changes in mitochondrial membrane potential (Δψm) were used as an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that in situ mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncouplers. Ca2+-induced Δψm dissipation was caused by MPTP opening because this process was inhibited by cyclosporin A. MPTP opening was enhanced by the pro-oxidant tert-butyl hydroperoxide.

Keywords: Tetraphenylphosphonium-selective electrode, Mitochondrial permeability transition pore, Hepatocytes

O2k-Network Lab: CZ Hradec Kralove Cervinkova Z


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Organism: Rat  Tissue;cell: Liver  Preparation: Permeabilized cells 



HRR: Oxygraph-2k