Lamberti 2016 Abstract MitoFit Science Camp 2016
|Development of a reference sample for HRR.|
Link: MiPNet 21.14
High-resolution respirometry (HRR) allows an efficient quantitative and qualitative analysis of mitochondrial energetics and the diagnostic assessment of function and dysfunction of oxidative phosphorylation (OXPHOS), known to be related to the occurrence of an increasing number of metabolic and other diseases. In the perspective of data harmonization between different laboratories and the establishment of a global data base on mitochondrial function (mtf), instrumental quality control combined with a standardized quality management system (QMSmtf) becomes crucial. This requires the availability of a reference sample which is functionally stable over time and across geographical space, as a basis of standard proficiency tests within and between reference laboratories.
The reference sample for respirometry has to be suitable for prolonged storage and for large scale production in order to allow broad distribution to laboratories worldwide. In contrast to other cell types, preliminary tests on HEK293T cells revealed that the viability of these cells was hardly affected even after prolonged storage and that several respiratory parameters slightly declined over time but did not show very dramatic reductions. Furthermore, in HEK293T cells even storage in dry ice for 48h appeared to be tolerated well. Therefore we conclude that HEK293T cells can be considered as promising candidates to serve as a valuable respirometric reference sample.
Labels: MiParea: Respiration
Organism: Human Tissue;cell: Kidney, HEK
Coupling state: LEAK, ROUTINE, OXPHOS, ET Pathway: F, N, S, Gp, CIV, NS, Other combinations, ROX HRR: Oxygraph-2k Event: A2 MitoFit Science Camp 2016
1-Oroboros Instruments, Innsbruck; 2-D. Swarovski Research Lab, Dept Visceral, Transplant and Thoracic Surgery, Innsbruck Med Univ, Austria. - [email protected]