Laouafa 2018 Adv Exp Med Biol
| Laouafa S, Roussel D, Marcouiller F, Soliz J, Bairam A, Joseph V (2018) Role of estradiol receptor beta (ERĪ²) on arterial pressure, respiratory chemoreflex and mitochondrial function in young and aged female mice. Adv Exp Med Biol 1071:115-27. |
Laouafa S, Roussel D, Marcouiller F, Soliz J, Bairam A, Joseph V (2018) Adv Exp Med Biol
Abstract: We tested the hypothesis that ERĪ² is involved in respiratory control in female mice. We used young adult (5-6 months-old) and aged (17-18 months-old) ERĪ²KO or wild-type controls (WT) female mice to assess arterial blood pressure (via a tail-cuff sensor) and indices of respiratory pattern (sighs and apneas - recorded by whole body plethysmography at rest). We also measured respiratory parameters at rest and in response to brief (<10 min) exposure to hypoxia (12% O2) or hypercapnia (5% CO2). Because ERĪ² is localized in mitochondria, and because estradiol and ERĪ² agonist increase mitochondrial O2 consumption, we assessed the mitochondrial respiration (with a high-resolution oxygraph system) and the in vitro activity of the complex I of the electron transfer chain in samples of brain cortex in aged wild-type and ERĪ²KO female mice. Compared to young WT mice, young ERĪ²KO mice had elevated arterial blood pressure, but similar ventilatory responses to hypoxia and hypercapnia. In old ERĪ²KO female mice compared to old WT mice, the arterial blood pressure was lower, the frequency of sighs was higher and the frequency of apneas was lower, and the hypoxic and hypercapnic ventilatory responses were reduced. In old ERĪ²KO mice mitochondrial respiration and complex I activities in the brain cortex were lower than in WT mice. We conclude that ERĪ² has age-specific effects on vascular and respiratory functions in female mice. ā¢ Keywords: Arterial pressure, Chemoreflex, Estradiol receptor beta, Female mice, Mitochondria, Old mice ā¢ Bioblast editor: Plangger M ā¢ O2k-Network Lab: CA Quebec Soliz J
Labels: MiParea: Respiration
Pathology: Aging;senescence
Stress:Hypoxia
Organism: Mouse
Tissue;cell: Nervous system
Preparation: Permeabilized tissue
Enzyme: Complex I
Coupling state: LEAK, OXPHOS, ET Pathway: N, S, CIV, ROX HRR: Oxygraph-2k
Labels, 2018-11
