Larsen 2012 CryoLetters
|Larsen S, Wright-Paradis C, Gnaiger E, Helge JW, Boushel RC (2012) Cryopreservation of human skeletal muscle impairs mitochondrial function. Cryo Letters 33:170-6.|
Abstract: Previous studies have investigated if cryopreservation is a viable approach for functional mitochondrial analysis. Different tissues have been studied, and conflicting results have been published. The aim of the present study was to investigate if mitochondria in human skeletal muscle maintain functionality after long term cryopreservation (1 year). Skeletal muscle samples were preserved in dimethyl sulfoxide (Me2SO) for later analysis. Human skeletal muscle fibres were thawed and permeabilised with saponin, and mitochondrial respiration was measured by high-resolution respirometry. The capacity of oxidative phosphorylation (OXPHOS) was significantly (P<0.05) reduced in cryopreserved human skeletal muscle samples. Cryopreservation impaired respiration with substrates linked to Complex I more than for Complex II (P<0.05). Addition of cytochrome c revealed an increase in respiration indicating cytochrome c loos from the mitochondria. The results from this study demonstrate that normal mitochondrial functionality is not maintained in cryopreserved human skeletal muscle samples.
• Keywords: Cryopreservation, High-resolution respirometry, Human skeletal muscle, Mitochondrial function, Permeabilised fibres
Labels: MiParea: Respiration, Instruments;methods
Stress:Cryopreservation, Ischemia-reperfusion Organism: Human Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS Pathway: N, S, NS HRR: Oxygraph-2k