Leguina-Ruzzi 2020 Biomolecules

From Bioblast
Publications in the MiPMap
Leguina-Ruzzi A, VodičkovÑ A, HolendovÑ B, Pavluch V, Tauber J, EngstovÑ H, DlaskovÑ A, Ježek P (2020) Glucose-induced expression of DAPIT in pancreatic β-cells. Biomolecules 10:E1026.

Β» PMID: 32664368 Open Access

Leguina-Ruzzi Alberto, Vodickova Anezka, Holendova Blanka, Pavluch Vojtech, Tauber Jan, Engstova Hana, Dlaskova Andrea, Jezek Petr (2020) Biomolecules

Abstract: Transcript levels for selected ATP synthase membrane FO-subunits-including DAPIT-in INS-1E cells were found to be sensitive to lowering glucose down from 11 mM, in which these cells are routinely cultured. Depending on conditions, the diminished mRNA levels recovered when glucose was restored to 11 mM; or were elevated during further 120 min incubations with 20-mM glucose. Asking whether DAPIT expression may be elevated by hyperglycemia in vivo, we studied mice with hyaluronic acid implants delivering glucose for up to 14 days. Such continuous two-week glucose stimulations in mice increased DAPIT mRNA by >5-fold in isolated pancreatic islets (ATP synthase F1Ξ± mRNA by 1.5-fold). In INS-1E cells, the glucose-induced ATP increment vanished with DAPIT silencing (6% of ATP rise), likewise a portion of the mtDNA-copy number increment. With 20 and 11-mM glucose the phosphorylating/non-phosphorylating respiration rate ratio diminished to ~70% and 96%, respectively, upon DAPIT silencing, whereas net GSIS rates accounted for 80% and 90% in USMG5/DAPIT-deficient cells. Consequently, the sufficient DAPIT expression and complete ATP synthase assembly is required for maximum ATP synthesis and mitochondrial biogenesis, but not for insulin secretion as such. Elevated DAPIT expression at high glucose further increases the ATP synthesis efficiency. β€’ Keywords: ATP synthase oligomers mitochondrial cristae morphology, USMG5/DAPIT, Glucose-induced expression, Glucose-stimulated insulin secretion, Membrane subunits of ATP synthase, Mitochondria β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: CZ Prague Jezek P

Labels: MiParea: Respiration, mtDNA;mt-genetics, Genetic knockout;overexpression 

Organism: Rat  Tissue;cell: Islet cell;pancreas;thymus  Preparation: Intact cells 

Regulation: Substrate  Coupling state: LEAK, ROUTINE, ET 

HRR: Oxygraph-2k 


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