Lytovchenko 2015 Abstract MiP2015
|Mitochondrial metabolite transport in cancer cells.|
Lytovchenko O, Porcelli AM, Kunji ERS (2015)
Carcinogenesis is accompanied by significant remodeling of cellular metabolic pathways, in particular those involving mitochondria. These alterations lead to corresponding changes in metabolite fluxes across the mitochondrial inner membrane. Although the changes in cancer cell metabolism are extensively studied, there are no systematically collected data on mitochondrial transport in these cells.
The mitochondrial inner membrane is not permeable for small hydrophilic molecules. The vast majority of the mitochondrial metabolite transport is mediated by proteins belonging to the mitochondrial carrier family. In this study, we have investigated the abundance and transport activities of mitochondrial carriers in several cancerous and non-cancerous cell lines under normoxic and hypoxic conditions by real-time PCR and Western blotting. Several proteins were significantly upregulated in cancer cells, indicating that their transported metabolites are of special importance for mitochondrial and cellular metabolism under these conditions.
These data give important insights into adaptation of mitochondria to the metabolic challenges of cancer cells. As transport processes are often rate-limiting steps of metabolic pathways, mitochondrial carriers overexpressed in cancer might represent a promising target for future anticancer therapies.
Labels: MiParea: mt-Membrane Pathology: Cancer Stress:Oxidative stress;RONS
Tissue;cell: Other cell lines
Event: A2, Poster MiP2015
1-Medical Research Council, Mitoch Biol Unit, Cambridge, UK; 2-Dept Pharmacy Biotech, Univ Bologna, Italy. - [email protected]