MiPNet09.12 Manual titrations of SUIT chemicals

                           

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MiPNet09.12 Manual titrations of SUIT chemicals

Manual titrations of SUIT chemicals with 2.0 mL and 0.5 mL chambers of the Oroboros Bioenergetics Analyzer. Mitochondr Physiol Network 09.12(21)

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Oroboros (2024-11-25) Mitochondr Physiol Network

Abstract: This document applies to

• Oroboros

» Oroboros NextGen-O2k • Oroboros O2k • Oroboros sole source

• Current Oroboros packages » NextGen-O2k Series XB and O2k Series J
• Current DatLab version » DatLab 8.2

• O2k-Network Lab: AT_Innsbruck_Oroboros

Note

Oligomycin: titrations should be performed by 1-2 µL steps (i.e. 5-10 nM experimental concentration of each step). Careful titration of oligomycin is necessary to determine the optimal inhibitory concentration (from 10 to 40 nM) as elaborated in Vernerova et al 2021 and Zdrazilova et al 2022 and to prevent inhibition of ET respiration as detailed in Ruas et al (2016) and Doerrier et al 2018.

Labels: MiParea: Instruments;methods 

Regulation: ADP, Inhibitor, Substrate, Uncoupler, Amino acid 

HRR: O2k-Manual 

O2k-chemicals and media, ADP, OroM3