Abstract: Simonovik B, Gnaiger E (2017) A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast. Mitochondr Physiol Network 15.09(03).
A mitochondrial reference assay (mtRA) was designed for high-resolution respirometry, to evaluate the reproducibility of cell respiration with baker’s yeast (Saccharomyces cerevisiae). Quality control is an integral part of quality assurance (QA), which is becoming increasingly important in high-resolution respirometry for basic and clinical research, as well as industrial applications. Quality control will ensure comparability of quantitative results obtained by different research groups.
• Keywords: Yeast: HRR Reference Assay
• O2k-Network Lab: AT Innsbruck Oroboros
Labels: MiParea: Respiration, Instruments;methods
Organism: Saccharomyces cerevisiae, Fungi
Coupling state: ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k, O2k-Protocol
- O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. - »Bioblast link«