MiPNet17.03 Shredder vs Fibers
Abstract: Doerrier C, Draxl A, Wiethuechter A, Eigentler A, Gnaiger E (2015) Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder. Mitochondr Physiol Network 17.03(04):1-9.
In the present study we compared mitochondrial function of permeabilized fibers and homogenate of heart muscle of mice. In addition, respiration of trout heart homogenate preparations were compared with permeabilized fibers, and the PBI-Shredder was successfully tested with preparation of trout liver.
• Keywords: Tissue homogenate, Shredder
Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP
Organism: Fishes Tissue;cell: Heart, Liver Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS HRR: Oxygraph-2k, O2k-Protocol
O2k-Demo, Mt-Preparation, Auxiliary, PBI-Shredder, O2k-Core